创伤性脑损伤后小鼠肠道Toll样受体4的表达  

Up-regulation of TLR4 in the gut of rats after traumatic brain injury

作　　者：丁可[1] 王汉东[1] 成惠林[1] 吴波[2] 杭春华[1] 史继新[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)神经外科,江苏南京210002 [2]南京大学医学院临床学院(南京军区南京总医院)病理科,江苏南京210002

出　　处：《肠外与肠内营养》2010年第4期243-246,共4页Parenteral & Enteral Nutrition

摘　　要：目的:探讨Toll样受体4(TLR4)在创伤性脑损伤后肠道的表达变化。方法:将小鼠随机分为对照组和脑损伤后4 h、24 h和7 d组,每组5只。观察小鼠脑损伤后肠黏膜组织的改变,用免疫组化技术观察TLR4蛋白表达,实时RNA检测肠道TLR4mRNA表达。结果:小鼠创伤后4 h肠黏膜出现病理改变,并逐渐加重。TLR4蛋白阳性细胞伤后4 h升高,24 h达到高峰,7 d下降。TLR4mRNA水平伤后4 h升高,24 h达到高峰,7 d下降。结论:小鼠创伤性脑损伤后,可引起肠道TLR4表达增高。Objective：The aim of the current study was to explore the alterations of TLR4 after traumatic brain injury. Methods： 20 Male ICR rats were randomly divided into four groups（5 rats each group）including control group without brain injury and traumatic brain injury groups（4,24 hours and 7 days）.All rats were decapitated at corresponding time point and mid-jejunum samples were taken.The intestinal mucosa structure was detected by histopathological examination.Immunohistochemistry and realtime RNA were used for detection of TLR4 expression in ileum samples.Results： After traumatic brain injury,the histopatholocal alterations of gut mucosa occurred at 4 hours and progressed to a serious state.Compared to control group,TLR4 was significantly up-regulated on the endothelia of microvessels in villous interstitium and lamina propria by 4 h following TBI and maximally expressed at 24 h post-injury.The endothelial TLR4 immunoreactivity in ileum mucosa still remained strong on 7 d post-injury.There was a very low mRNA expression of TLR4 in the gut of control rats and significantly increased at 4 h following TBI（P〈0.05 vs control）,peaked at 24 h（P〈0.01）and remained elevated on 7 d post-injury.Conclusion： TBI can induce an immediate and persistent up-regulation of TLR4 expression in the intestine.Inflammatory response mediated by increased TLR4 expression may play an important role in the pathogenesis of acute gut mucosal injury following TBI.

关 键 词：TOLL样受体4 创伤性脑损伤 肠黏膜损伤 

分 类 号：R651.15[医药卫生—外科学]