鱼腥草素钠对心室重构大鼠心肌信号转导通路和内皮素-1mRNA表达的影响  

Effects of Sodium Houttuyfonate on Myocardial Signal Transduction and Gene Expression of Endothelin-1 in Rats with Ventricular Remodeling

作　　者：高建平[1] 陈长勋[1] 吴琦[1] 顾伟梁[1] 贺静[1] 刘莹[1] 

机构地区：[1]上海中医药大学中药学院,上海201203

出　　处：《上海中医药大学学报》2010年第4期55-59,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委科研基金项目(07CZ040)

摘　　要：目的:研究鱼腥草素钠对心室重构大鼠模型心肌信号转导通路和内皮素-1(ET-1)mRNA表达的影响。方法:采用不完全结扎腹主动脉制备压力超负荷性大鼠心室重构模型。造模3d后存活动物随机分为模型组,鱼腥草素钠小、大剂量组(50、100 mg/kg)和卡托普利组(40 mg/kg),同时设假手术组。各组动物每天灌胃给药或饮用水1次,连续4周。免疫组织化学法测左室心肌组织中蛋白激酶C(PKC)和p38丝裂原激活的蛋白激酶(p38 MAPK)表达量;实时定量逆转录-聚合酶反应测定左室心肌组织中ET-1 mRNA表达水平。结果:与假手术组比较,模型组大鼠心肌组织中ET-1 mRNA基因表达量明显提高,PKC和p38 MAPK蛋白表达量增高(P<0.05)。与模型组比较,大剂量鱼腥草素钠(100 mg/kg)和卡托普利具有降低ET-1 mRNA表达的趋势(0.05<P<0.1),鱼腥草素钠和卡托普利明显减少心肌PKC和p38 MAPK表达(P<0.05)。结论:鱼腥草素钠抗心室重构可能与抑制ET-1 mRNA的表达,调节PKC和p38 MAPK等信号通路有关。Objective ： To study the effects of sodium houttuyfonate on myocardial signal transduction and gene expression of endothelin-1 （ET-1） in rats with ventrieular remodeling, Methods： Pressure overloaded ventricular remodeling model was induced by abdominal aortic handing （AAB） in rats. The survival rats were randomly divided into AAB group, low （50mg/kg） and high （ 100 mg/kg） dose sodium houttuyfonate group and captopril （40 mg/kg） group. Meanwhile the sham-operated group was established. All the rats were intragastrically administered once a day for four weeks. At the end of the experiment, the protein expression levels of protein kinase C （PKC） and p38 mitogen-aetivated protein kinase （p38 MAPK） in left ventricle were detected with immunohistochemical analysis. The gene expression of ET-1 mRNA in left ventricle was detected by real-time reverse transcription polymerase chain reaction （ RT-PCR ）. Results： The levels of ET-lmRNA, PKC and p38 MAPK protein expression were higher in AAB group than in sham-operated group （P 〈 0.05 ）. Compared with the AAB group, high dose sodium houttuyfonate （100 mg/kg） or captopril group showed a tendency to decrease ET-1 mRNA expression （0.05 〈 P 〈 0.1 ）, and sodium houttuy-fonate and captopril also decreased the protein expression of PKC and p38 MAPK significantly （ P 〈 0.05 ）. Conclusion ： The beneficial effects of sodium houttuyfonate on ventricular remodeling are at least in part associated with decreasing the expression of the ET-1mRNA in ventricular tissue and have connection with the signal pathways of PKC and p38 MAPK. It also restrained PKC and p38 MAPK pathways.

关 键 词：鱼腥草素钠 心室重构 基因表达 蛋白表达 

分 类 号：R285.5[医药卫生—中药学]