高效液相色谱法测定设备淋洗液中头孢唑啉残留含量  

Determination of Residue Level of Cefazolin in the Elutriant of Equipment by HPLC

作　　者：孙志嘉[1] 张晓影[1] 

机构地区：[1]哈药集团制药总厂,150086

出　　处：《黑龙江医药》2010年第4期503-505,共3页Heilongjiang Medicine journal

摘　　要：目的:测定设备淋洗液中头孢唑啉残留量。方法:采用十八烷基硅烷键合硅胶为填充剂,以磷酸氢二钠和枸橼酸溶液〔取磷酸氢二钠1.33g与枸橼酸1.12g加水溶解稀释成1000mL〕-乙腈(88:12)为流动相,检测波长254nm,流速0.8mL/min,柱温40℃。结果:头孢唑啉在0.005963mg/mL～0.01391mg/mL范围有良好的线性关系(r为0.9997),检测限为9.938×10-5mg/mL,平均回收率为98.5%;RSD为0.69%。结论:该方法简便,重现性好,专属性强,为检测设备淋洗液中头孢唑啉残留含量提供了可靠的方法。The method can be used for the determination of residue level of Cefazolin in the elutriant of equipment,simple 、rapid、sensitive and specific,detectability was 9.938×10^-5mg/mL,the average recoveries rate was 98.7%;RSD=0.69%.

关 键 词：头孢唑啉 设备淋洗液 残留含量 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学]