60%氧暴露对新生小鼠一般状态及肺病理形态学的影响  

Effects of 60% oxygen on the morphologic characteristics in lung of neonatal mouse

作　　者：张志梅[1,2] 尉进茜[1] 封志纯[1] 

机构地区：[1]北京军区总医院附属八一儿童医院,100070 [2]山西医科大学

出　　处：《实用医技杂志》2010年第8期706-708,共3页Journal of Practical Medical Techniques

基　　金：国家自然科学基金(30772036)

摘　　要：目的探讨中浓度氧(60%O2)暴露对新生小鼠发育中肺的影响。方法 30只新生雌性KM小鼠,随机分为实验组和对照组,每组15只,实验组置于封闭氧箱中(60%O2),对照组置于空气中(21%O2),记录吸氧后2、7、14、21、28d小鼠体质量、肺组织病理改变以及辐射状肺泡计数(RAC)。结果实验组吸氧7d后体质量明显降低(P<0.05),苏木素-伊红染色下正常肺泡结构消失、肺泡融合、肺泡间隔增厚,RAC较对照组明显减少(P<0.01)。结论持续较高浓度氧吸入可致新生小鼠生长障碍和肺泡化阻滞,出现类似人类支气管肺发育不良的肺部改变。Objective To determine the effects of moderate hyperoxic exposure （60%） on neonatal mouse lung. Methods Thirty female KM mice were randomly divided into two groups： the normoxia group （21% O2, n=lS） and hyperoxia group （60% O2, n=15 ）. Body weight were recorded, histopathology examination of the lung tissue and radical alveoli count （RAC） were carried out. Results Hyperoxia-treated mice had lower body weight than those in normoxia group 7 days after oxygen uptaking （P〈0.05）. The results of HE assay of the hyperoxia group showed that the structure of normal alveoli disappeared, alveolar were fused andalveolar septum were thickened. The RAC value was significantly decreased in the hyperoxia group （P〈0.01）. Conclusion Long-term exposure on high concentration oxygen can result in impediment to growth and impairment of lung development in newborn mouse, which show morphologic changes of lung similar to human bronchopulmonary dysplasia.

关 键 词：氧吸入疗法 支气管肺发育不良 肺损伤 发育 

分 类 号：R722.1[医药卫生—儿科]