共轭亚油酸c9,t11-CLA及t10,c12-CLA对成骨细胞PPARγ2及骨代谢相关基因表达的影响  

Influence of conjugated linoleic acids (c9,t11-CLA and t10,c12-CLA) on mRNA expression of PPARγ2 and osteoblastogenesis-related markers in osteoblastic cells

作　　者：李丽婷[1] 朱亦堃[1] 郗光霞[1] 史书红[1] 李兴[1] 赵宝珍[1] 

机构地区：[1]山西医科大学第二医院内分泌科,太原030001

出　　处：《中国药物与临床》2010年第8期858-861,共4页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关计划项目(200911)

摘　　要：目的观察不同浓度共轭亚油酸(c9,t11-CLA及t10,c12-CLA)干预后大鼠成骨细胞过氧化物酶体增殖物激活受体(PPAR)γ2及骨代谢相关基因核因子-κB活化受体配体(RANKL)、碱性磷酸酶(ALP)、骨保护素(OPG)mRNA表达水平的变化,探讨c9,t11-CLA及t10,c12-CLA对成骨细胞PPARγ2及骨代谢相关基因表达的影响。方法体外培养大鼠成骨细胞,分别加入c9,t11-CLA和t10,c12-CLA(终浓度均为0、12.5、25、50μmol/L)干预24h后,反转录聚合酶链反应(PCR)法检测成骨细胞PPARγ2、RANKL、ALP、OPG mRNA表达水平,比较不同浓度c9,t11-CLA及t10,c12-CLA对上述基因表达的影响。组间比较采用单因素方差分析。结果①不同浓度c9,t11-CLA呈剂量依赖性上调成骨细胞RANKL、OPG mRNA的表达水平,组间比较差异有统计学意义(P<0.05,P<0.01);c9,t11-CLA上调ALP mRNA的表达水平与剂量无关,而其对PPARγ2 mRNA的表达影响不明显。②不同浓度t10,c12-CLA呈剂量依赖性上调RANKL和OPG mRNA的表达水平,组间比较差异有统计学意义(P<0.05,P<0.01);且呈非剂量依赖性上调ALPmRNA的表达,下调PPARγ2mRNA的表达,与对照组比较差异有统计学意义(P<0.05,P<0.01)。结论 c9,t11-CLA及t10,c12-CLA可能通过促进成骨细胞标记物基因表达有利于骨形成,可能为骨质疏松的治疗提供新思路。Objective To observe the effects of conjugated linoleic acids （c9,t11-CLA and t10,c12-CLA） at various dosage on mRNA expression of peroxisome proliferator activated receptorsγ2 （PPARγ2）, receptor activator of NF-κB ligand （RANKL）, alkaline phosphatase （ALP） and osteoprotegerin （OPG） of osteoblastic cells, and to study the effects of c9,t11-CLA and t10,c12-CLA on bone metabolism. Methods Osteoblastic cells of Wistar rats were cultured in vitro and the fourth generation cells were selected to be cultured with c9, t11-CLA or t10, c12-CLA at different concentrations （the final concentration was 0, 12.5, 25, 50μmol/L, respectively） for 24h. RT-PCR was performed to semi-quantitatively determine and compared the mRNA expression of PPARγ2, RANKL, ALP and OPG as affected by different levels of c9, t11-CLA and t10, c12-CLA. Results Semi-quantitative RT-PCR analysis showed that c9,t11- CLA up-regulated the mRNA expression of RANKL and OPG in a dose-dependent manner （P0.05, P0.01, by interclass comparison） as well as the ALP mRNA expression albeit in a non-dose-dependent manner, but had no effect on the mRNA expression of PPARγ2. t10,c12-CLA up-regulated the mRNA expression of RANKL and OPG in a dose- dependent manner （P0.05, P0.01, by interclass comparison） as well as the ALP mRNA expression albeit in a non- dose-dependent manner, but down-regulated the expression of PPARγ2 （P0.05, P0.01, compared with controls）. Conclusion c9,t11-CLA and t10,c12-CLA may promote the expression of osteogenic genes and thereby facilitate osteogenesis. These findings tentatively suggested a possible beneficial effect on bone formation.

关 键 词：过氧化物酶体增殖物激活受体 亚油酸类 共扼 成骨细胞 骨质疏松 

分 类 号：R580[医药卫生—内分泌]