小鼠精子和球形精子细胞显微注射受精研究  

FERTILIZATION BY MICROINJECTION OF SPERMATOZOON AND ROUND SPERMATID INTO OOCYTE IN THE MOUSE

作　　者：李子义[1,2] 谭景和[1,2] 孙兴参[1,2] 刘忠华[1,2] 周琪[1,2] 贺桂馨[1,2] 

机构地区：[1]东北农业大学生物工程系 [2]解放军农牧大学组织学胚胎学教研室

出　　处：《解剖学报》1999年第2期147-151,共5页Acta Anatomica Sinica

基　　金：黑龙江省科委资助

摘　　要：目的研究动物受精机理。方法以昆明白鼠为实验动物，利用显微注射技术，对精子和球形精子细胞的显微注射受精进行了研究。结果１．带下注射受精，注射４～６个精子的卵的受精率（３２．５％）和卵裂率（２５．０％）显著高于注射单个精子的卵的受精率和卵裂率（分别为１２．５％和６．３％）（Ｐ＜０．０５）。２．带下注射４～６精子后，有第１极体卵母细胞的存活率（６９．６％）、受精率（３２．５％）和卵裂率（２５．０％）分别高于无第１极体卵母细胞的存活率、受精率和卵裂率（分别为６４．４％、２０．７％和１３．８％），但两者无显著差异（Ｐ＞０．０５）。３．胞质内精子注射卵的存活率（２１．４％）极显著低于带下注射卵的存活率（６９．６％）（Ｐ＜０．０１）；而胞质内精子注射卵的受精率（３５．５％）、卵裂率（３２．３）％和囊胚率（２０．０％）则高于带下注射卵的受精率（３３．３％）、卵裂率（２５．０％）和囊胚率（１０．０％），但无显著差异（Ｐ＞０．０５）。４．将球形精子细胞注入经电活化处理的卵母细胞的透明带下，注射卵的存活率为９０．５％，电融合率为３４．６％，２－细胞分裂率为２８．３％。结论１．注射精子的数量对小鼠带下注射的受精率和卵裂率有显著影响?Objective\ To study fertilization mechanism in mammals and provide essential data for treating human male infertility. Methods Using microinjection technique, the fertilization by microinjection of spermatozoa and round spermatids into oocytes in the mouse was systematically studied. Results 1 Following subzonal injection of spermatozoa into occytes, the fertilization rate (32 5%) and cleavage rate(25 0%) in the oocytes injected with 4 6 spermatozoa were significantly higher ( P <0 05) than those (12 5% and 6 3%, respectively) in the oocytes injected with a single spermatozoon. 2 Following subzonal injection of 4 6 spermatozoa into the oocytes, the survival rate (69 6%), fertilization rate (32 5%) and cleavage rate (25 0%) in those oocytes with polar body 1 (PB1) were higher than those (64 4%, 20 7% and 13 8%, respectively) in the oocytes without PB1, but the difference was not significant ( P >0 05). 3 The survival rate (21 4%) in the oocytes injected with a single spermatozoon into the cytoplasm was significantly lower ( P <0 01) than that (69 0%) in the oocytes injected with 4 6 spermatozoa into the perivitelline space (PVS). The fertilization rate (35 5%), cleavage rate (32 2%) and blastocyst rate (20 0%) in those oocytes injected with a single spermatozoon into the cytoplasm of oocytes were higher than those (33 3%, 25 0% and 10 0%, respectively) in the oocytes injected with 4 6 spermatozoa into the PVS, but they did not differ significantly( P >0 05). 4 Following subzonal injection of a round spermatid into the oocyte, the survival rate, electrofusion rate and 2 cell rate were 90 5%, 34 6% and 28 3%, respectively.Conclusions 1 The fertilization rate and cleavage rate in the oocytes were significantly affected by the number of subzonal injection of spermato

关 键 词：精子 球形精子细胞 显微注射 小鼠 受精 

分 类 号：Q492.6[生物学—生理学]