大黄素对胰腺癌细胞BXPC-3的体内抑制作用  

Anti-tumor activity of emodin on human pancreatic cancer cell line BXPC-3 in vivo

作　　者：童洪飞[1] 林海舵[1] 张伟[1] 陈辉[1] 林胜璋[1] 

机构地区：[1]温州医学院附属第二医院,温州325027

出　　处：《中华中医药杂志》2010年第8期1211-1214,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金(No.Y2080708);浙江省中医药管理局重点项目(No.2008ZA015)~~

摘　　要：目的:探讨大黄素(Emodin,EMO)在体内对胰腺癌BXPC-3细胞移植瘤的体内抑制作用,并探讨其初步机制。方法:建立胰腺癌裸鼠移植瘤模型,分为对照组(NS)、大黄素组(EMO)和吉西他滨组(Gemcitabine,GEM)3组,各组通过腹腔注射作用2周后,检测实验前后裸鼠体质量变化及抑瘤率,免疫组化检测细胞增殖因子ki-67的表达,TUNEL法检测移植瘤组织的细胞凋亡。结果:EMO组(25.1±1.58)g、和GEM(25.6±1.47)g组的动物体质量与对照组(27.0±1.64)g相比,无显著性差异;EMO组和GEM组的抑瘤率分别为38.46%和44.23%,免疫组化检测ki-67结果显示,与对照组IOD(219.5±17.98)相比,EMO组IOD(146.6±11.57)和GEM组IOD(139.5±12.55)明显减小(P<0.01),EMO组和GEM组间比较差异无统计学意义。TUNEL检测结果显示,与对照组IOD值(23.6±6.02)相比,EMO组IOD值(65.7±4.97)和GEM组IOD值(67.4±5.59)明显增大(P<0.01);EMO组和GEM组间比较差异无统计学意义。结论:大黄素可抑制胰腺癌细胞株BXPC-3细胞在裸鼠体内的生长;此作用机制可能为抑制肿瘤细胞增殖及促进肿瘤细胞的凋亡。Objective： To investigate the effect and mechanism of emodin （EMO） on human pancreatic cancer cell line BxPC-3 in vivo. Methods： After the pancreatic cancer model in nude mice was established, the mice were divided into four groups： control group （NS 0.2mL/d by i.p. injection）, EMO group （EMO 40mg？kg-1？d-1 by i.p. injection）, and Gemcitabine （GEM） group （GEM 80mg/kg, twice/week by i.p. injection） with 8 mice each group. After 2 weeks of administration, the mice were sacrificed, detected the body-weight change of nude nice before and after the experiment, and recorded the growth inhibition rate of tumor （TGI）. Immunohistochemical （IHC） staining for ki-67 and terminal deoxynucleotidyl transferase-mediated nicked labeling assay （TUNEL） were undertaken to detect the cell proliferation and cell apoptosis in tumor tissue in xenograft nude mice. Results： The inter-group comparisons in body-weight of nude mice showed no significant difference in comparing group NS（27.0±1.64）g with group EMO（25.1±1.58）g and GEM（25.6± 1.47）g.The EMO group was 38.46%, the GEM group was 44.23%. The inter-group comparisons in immunohistochemical analysis of ki-67 showed significant difference in comparing group NS IOD（219.5±17.98） with group EMO IOD（146.6± 11.57）and GEM IOD（139.5±12.55）, （P0.01）, and the expression of ki-67 in EMO and GEM groups were lower than that in NS group, P0.05; while the expression of ki-67 between EMO and GEM groups showed no significant difference. The inter-group comparisons in TUNEL analysis showed significant difference in comparing group NS IOD（23.6±6.02） with group EMO IOD（65.7±4.97）and GEM IOD（67.4±5.59）, （P0.01）, and the TUNEL-positive cells in EMO and GEM groups were higher than that in NS group; while the TUNEL-positive cells between EMO and GEM groups showed no significant difference. Conclusion： Emodin can inhibit the growth of pancreatic cancer cells BXPC-3 in nude mice; and this affection was achieved by s

关 键 词：胰腺癌 大黄素 凋亡 裸鼠移植瘤 

分 类 号：R285.5[医药卫生—中药学]