雄黄微生物炮制及其药效与毒性的重新评价  

Processing of realgar and re-evaluation on medicinal efficacy and toxicity

作　　者：张景红[1,2] 李红玉[1] 

机构地区：[1]兰州大学生命学院生物技术开发试验室,兰州730000 [2]福建省华侨大学分子药物研究所天然药物研究中心,泉州362021

出　　处：《中华中医药杂志》2010年第8期1321-1323,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：甘肃省科技攻关项目(No.2GS064-A43-019-02);福建省自然科学基金(No.2008J0102);泉州市科技项目(No.2008Z21);华侨大学高层次人才科研启动费项目(No.08BS105)~~

摘　　要：目的:制备雄黄微生物炮制液,观察其药效和毒性的变化。方法:利用氧化亚铁硫杆菌制备雄黄炮制液;以常规炮制雄黄作为对照,以人多发性骨髓瘤细胞株RPMI8226为细胞模型,应用噻唑蓝(MTT)法和流式细胞仪(FCM),检测两种药物对细胞增殖与细胞周期的影响;以离子发射原子光谱仪法(ICP-AES),测定其中砷在小鼠血液和组织中分布的浓度变化。结果:与常规炮制雄黄相比,雄黄微生物炮制后,溶解度提高,用药剂量显著降低,对细胞抑制凋亡作用增强,蓄积量显著降低,在大鼠各组织器官内几乎未检测出砷的分布。结论:微生物新法炮制雄黄后,药效增强、砷蓄积毒性降低,为科学炮制雄黄提供了新的思路。Objective： To prepare the processing solution of realgar by bacteria and to explore the change of pharmacodynamic action and toxicity. Methods： The processing solutions of realgar were prepared using the strain thiobacillus ferroxidans, the realgar powder processed by normal method was selected as control. The effects on RPMI8226 cell proliferation of them were investigated by methyl thiazolyl terazolium （MTT）,cell cycle distribution was determined by FCM in RPMI8226 cell. The distribution of arsenic concentration in blood and tissues including heart, liver, spleen, lung, and kidney in the samples were determined by Inductively Coupled Plasma-atomic Emission Spectrometry （ICP-AES）. Results： Compared with control group, the solubility rate and the induced apoptotic rate increased, and dosage significantly reduced, while arsenic was hardly found in the tissue in processing group by bacteria. Conclusions： The processing group by bacteria may be enhanced medicinal efficacy and inhibited the toxicity in realgar. It’s intending to provide new approaches for scientific processing realgar.

关 键 词：微生物 炮制 雄黄 药效 毒性 

分 类 号：R283[医药卫生—中药学]