低氧训练对大鼠骨骼肌血管新生的影响  

Effect of Hypoxic Training on Skeletal Muscle Angiogenesis of Rats

作　　者：邹志兵[1] 郑澜[2] 

机构地区：[1]广西民族大学相思湖学院,广西南宁530008 [2]湖南师范大学体育学院,湖南长沙410081

出　　处：《广州体育学院学报》2010年第4期51-54,69,共5页Journal of Guangzhou Sport University

基　　金：国家自然科学基金项目(30671011)资助

摘　　要：目的:研究不同的低氧训练模式对大鼠骨骼肌组织血管生成的作用。方法:将健康雄性SD大鼠60只,按体重随机分为6组,每组10只。运动组采用10周递增负荷跑台运动训练,每周训练6天,运动量由第1周的速度为15m/min、持续时间为25min递增至第10周速度为28m/min、持续时间为50min,低氧训练组每周二、四、六在相当于海拔1500m的低氧环境中训练,一、三、五在常氧下训练。并且在低氧环境中居住,低氧环境由第1周相当于海拔1800m递增至第10周相当于海拔3600m。应用免疫组织化学、显微图象对CD34的阳性表达进行定性和定量分析。结果:CD34可较好标记骨骼肌组织微血管,低氧训练组有丰富的微血管新生。结论:单纯低氧不能显著增加骨骼肌组织的血管生成,运动能使骨骼肌组织血管产生适应性变化,当低氧与运动两种因素同时介入,血管生成丰富。Objective： This paper studied the effect on different hypoxic training pattern on angiogenesis of skeletal muscle. Methods： 60 male SD rats were randomly divided into six groups, 10 rats each. 10 weeks progressive treadmill exercise program were designed with intensity starts from 15m/min for 25min at the first week to 28m/min for 50min in the last week and hypoxia degree from 1800m at the first week to 3600m in the last week. And the hypoxic training groups were trained in the hypoxia cabin, oxygen concentration equal to 1500m height every week2,4,6, every week1,3,5 were trained in normal oxygen concentration environment. Using the qualitative and quantitative analysis by immunity histochemistry and the micro image analysis to study the expression of CD34. Results： CD34 may be a good marker of skeletal muscle Angiogenesis, hypoxia training group a large number of CD34 protein expression. Conclusion： Hypoxia alone can not be increased in skeletal muscle angiogenesis, exercise can produce adaptive changes, but hypoxic training can increase angiogenesis significantly.

关 键 词：低氧训练 骨骼肌组织 血管生成 

分 类 号：G804.7[文化科学—运动人体科学]