拮抗内皮素生物学效应对甘油所致大鼠急性肾功能衰竭的影响  

Effects of antagonizing the biological actions of endothelin on glycerol-induced acute renal failure

作　　者：夏晓红[1,2] 申玉学[1,2] 石纪才 于占久[1,2] 何瑞荣 

机构地区：[1]河北省医科院实验医学研究所 [2]河北医科大学基础医学研究所

出　　处：《中国病理生理杂志》1999年第4期306-308,共3页Chinese Journal of Pathophysiology

基　　金：河北省自然科学基金

摘　　要：目的和方法：在甘油致大鼠急性肾功能衰竭（ＡＲＦ）模型上，观察外源内皮素（ＥＴ）以及心房钠尿肽（ＡＮＰ）、硝苯吡碇（Ｎｉｆ）和ＮＯ前体Ｌ－精氨酸（Ｌ－Ａｒｇ）的作用。结果：甘油致大鼠ＡＲＦ后２４ｈ，肾功能明显受损，血清尿素氮（ＳＵＮ），肌酐（Ｓｃｒ），丙二醛（ＭＤＡ），肾皮质钙含量及血浆内皮素（ＰＥＴ）水平均明显高于正常对照组（Ｐ＜０００１）。外源性ＥＴ可加重ＡＲＦ大鼠肾损伤，使ＳＵＮ、Ｓｃｒ、ＭＤＡ、ＰＥＴ及肾皮质钙含量显著增加，而ＡＮＰ、Ｎｉｆ和Ｌ－Ａｒｇ则使ＡＲＦ大鼠ＳＵＮ、Ｓｃｒ、ＭＤＡ、ＰＥＴ及肾皮质钙含量明显降低，肾组织ｃＧＭＰ含量增加，减轻肾小管损伤。结论：ＥＴ对ＡＲＦ的发生发展具有重要意义，ＡＮＰ、Ｎｉｆ和Ｌ－Ａｒｇ可通过不同途径拮抗ＥＴ的生物学效应，从而对ＡＲＦ的防治起到积极的作用。AIM and METHODS:The effects of atrial natriuretic peptide (ANP), nifidepine (Nif) and L-arginine (L-Arg), the physiological NO precursor were observed on a model of glycerol-induced acute renal failure (ARF) of rat, respectively. RESULTS:The impairment of renal function was exaggerated, as manifested by significant increases in serum urea nitrogen(SUN),serum creatinine (Scr), MDA, P ET and the contents of calcium of renal cortex. Exogenous endothelin (ET) might exaggerate the renal injury of ARF rats, and significantly increase the levels of SUN, Scr, MDA, P ET and calcium contents of renal cortex, while ANP, Nif and L-Arg could decrease the level of SUN, Scr, MDA, P ET and calcium contents of renal cortex, and increase contents of cGMP of renal tissue, and attenuate the renal tubular injury.CONCLUSION:ET have the potential pathogenetic role in the glycerol induced ARF. ANP, Nif and L-Arg could block the biological actions of ET via different ways, thereby affording the protective action against ARF.

关 键 词：心钠素 硝苯地平 精氨酸 肾功能衰竭 内皮素 

分 类 号：R692.502[医药卫生—泌尿科学]