氟致小鼠睾丸组织形态结构损伤的观察  

Morphologic Observation of Mouse Testicular Tissue Lesions Induced by Fluorine

作　　者：吴培福[1] 曲伟杰[2] 郭爱伟[1] 郭经荣[1] 

机构地区：[1]西南林学院保护生物学学院,云南昆明650224 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《畜牧与饲料科学》2010年第4期20-22,共3页Animal Husbandry and Feed Science

基　　金：云南省高端科技人才引进计划项目(2009CI125);云南省教育厅科学研究基金(09Y0289)

摘　　要：选取20只20日龄的昆明小鼠,随机分为4组,分别给予含0,50,100和150 mg/L氟化钠的饮水120 d,通过石蜡组织切片观察氟对睾丸的损伤作用。结果发现,小鼠饮用含有氟化钠的水后,曲细精管管壁断裂、结构排列紊乱;生精细胞、次级精母细胞从生精上皮脱落;精母细胞胞质和胞核中出现大量的空泡样病变,且细胞的微核率增加;精子出现畸形。这说明氟对睾丸组织结构具有明显的损伤作用。A total of 20 Kunming mice at the age of 20 days were divided into four groups randomly.The mice in different group were respectively fed with water containing 0,50,100 and 150 mg/L sodium fluoride for 120 d.The lesions of testicular tissue induced by fluoride were verified using paraffin-tissue sections.The results showed that fluoride had great effects on testicular tissue,which were as follows：the wall of convoluted tubule was disrupt,the structure of that was disordered;sperm cells and secondary spermatocytes were shed from seminaferous epithelium;vacuolated changes were found from cytoplasm and nucli of spermatocytes;cellular micronuclei frequency was higher;and deformity sperms were also found.

关 键 词：氟 昆明小鼠 睾丸 形态结构 

分 类 号：Q492.4[生物学—生理学]