鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞p53和Bcl-2表达影响的实验研究  

The Rat's Blood Serum of Biejia Decoction for Gene Expression of Human's Liver HEPG Cell p53 and Bcl-2 to Study

作　　者：王丹[1] 宋昊[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2010年第7期1507-1509,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:研究鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞p53和bcl-2表达的影响。方法:将实验大鼠随机分为4组,生理盐水灌胃组、鳖甲煎丸煎剂灌胃组、西药组、中西医联合用药组。无菌条件下从大鼠后腔静脉采血,分离血清。将同组大鼠血清混匀,冷藏备用。待人肝癌HEPG2细胞贴壁生长状态良好,加入含药大鼠血清及对照组大鼠血清,培养后取材制作标本。对标本进行含药血清作用的Western-blot蛋白表达分析即p53蛋白表达和Bcl-2蛋白的表达。结果:鳖甲煎丸煎剂灌胃组、西药组、中西医联合用药组p53蛋白表达增强,与灌服生理盐水组相比较有明显差异,差别有统计学意义(P<0.05)。鳖甲煎丸煎剂灌胃组、西药组、中西医联合用药组p53蛋白表达增强,三者无明显差异(P>0.05)。从数据上看,含药大鼠血清对p53蛋白表达增强的作用,中西药结合用药组最好,鳖甲煎丸灌胃组次之,最后是西药组。鳖甲煎丸煎剂灌胃组、西药组、中西医联合用药组Bcl-2蛋白表达减弱,与灌服生理盐水组相比较有明显差异,差别有统计学意义(P<0.05)。鳖甲煎丸煎剂灌胃组、西药组、中西医联合用药组Bcl-2蛋白表达减弱,三者无明显差异(P>0.05)。从数据上看,含药大鼠血清对Bcl-2蛋白表达减弱的作用,中西药结合用药组最好,鳖甲煎丸灌胃组次之,最后是西药组。结论:鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞p53蛋白表达有增强作用,对Bcl-2蛋白表达有减弱作用。Objective： Discuss the effect of the rat＇s blood serum of Biejia decoction for gene expression of human＇s liver HIPG cell p53 and Bcl-2.Methods： The rats for experiment were separated as physiological saline group,Biejia decoction group,DDP group,Biejia decoction and DDP group.Take blood from the posterior vena cava of the rat under aseptic conditions,Separate the serum.Mix the serum of the same group and refrigeration.When human＇s liver HEPG cells were in good condition,including the serum of the rat each group.After culturing,make the specimen.Make the protein expression of the rats＇ serum（p53 gene and Bcl-2 gene）.Results： The expression of p53 gene of Biejia decoction group,DDP group,Biejia decoction and DDP group are all increased.Have the marked difference with the physiological saline group（P〈0.05）.The expression of p53 gene of Biejia decoction group,DDP group,Biejia decoction and DDP group are all increased.They are have no difference（P〈0.05）.From the data,the expression of p53 gene of the rat＇s blood serum of medicaments for gene expression of human＇s liver HIPG cell is increased.Biejia decoction and DDP group is the best,Biejia decoction group is the better and DDP group is good.The expression of Bcl-2 gene of Biejia decoction group,DDP group,Biejia decoction and DDP group are all decreased.Have the marked difference with the physiological saline group（P〈0.05）.The expression of Bcl-2 gene of Biejia decoction group,DDP group,Biejia decoction and DDP group are all decreased.They are have no difference（P〈0.05）.From the data,the expression of Bcl-2 gene of the rat＇s blood serum of medicaments for gene expression of human＇s liver HIPG cell is decreased.Biejia decoction and DDP group is the best,Biejia decoction group is the better and DDP group is good.Conclusion： The expression of p53 gene of the rat＇s blood serum of Biejia decoction for gene expression of human＇s liver HIPG cell is increased and Bcl-2 gene is decreased.

关 键 词：鳖甲煎丸 肿瘤 大鼠 人肝癌HEPG2细胞 P53基因 BCL-2基因 

分 类 号：R285.5[医药卫生—中药学]