真菌漆酶对蒽醌和偶氮类活性染料的脱色  

Decolorization of reactive dyes of anthraquinone and azo by fungal laccase

作　　者：朱林[1] 洪宇植[1] 方泽民[1] 刘娟娟[1] 肖亚中[1] 

机构地区：[1]安徽大学生命科学学院生物工程研究中心,合肥230039

出　　处：《中国科技论文在线》2010年第3期224-227,共4页

基　　金：国家自然科学基金资助项目(30770058);高等学校博士学科点专项科研基金资助项目(20093401110006);安徽省优秀青年科技基金资助项目(08040106908)

摘　　要：采用酶学技术脱色/脱毒纺织染料是环境生物技术领域研究的热点。以真菌5319漆酶粗酶液对2种蒽醌类和3种偶氮类纺织活性染料进行脱色,实验研究了温度、pH、染料浓度、给酶量和介体对脱色效率的影响。在pH4.5、给酶量10U/L、45℃条件下,无介体存在时,漆酶对蒽醌类染料活性艳蓝X-BR和K-GR的脱色率均超过80%,而对偶氮类染料活性深蓝M-2GE、活性红KD-8B和活性艳橙K-7R的脱色效果不佳,但在介体1-羟基苯并三唑或丁香酸甲酯存在时,漆酶对偶氮染料的脱色率均超过60%。真菌5319漆酶在这些工业纺织活性染料脱色方面具有潜在应用价值。Decolorization and detoxification of textile dyes by enzymes attracts attention in environmental biotechnology. In this study, we used the crude laccase from fungus No.5319, a newly isolated strain with high production of laccase, to decolorize 2 anthraquinone dyes and 3 azo dyes. The temperature, pH, dye concentration and laccase amount optimal for decolorization were tested. Over eighty percent of anthraquinone dyes （Reactive Brilliant Blue X-BR and K-GR） of 0.4 g/L were decolorized within 5 h by 10 U/L of laccase at pH 4.5 and 45 ℃ in the absence of mediators. In reverse, the decolorization of three azo dyes, Reactive Deep Blue M-2GE, Reactive Red KD-8B and Reactive Brilliant Orange K-7R, was relatively low without mediators. However, an addition of the mediator of 1-hydroxybenzotriazole or methyl-syringate remarkably improved the decolorization to over 60%. The results suggested that it is reseanable to use fungal laccase to decolorize the reactive dyes redundant in textile waste water.

关 键 词：漆酶 蒽醌染料 偶氮染料 介体 脱色 

分 类 号：X703[环境科学与工程—环境工程]