七叶树愈伤组织诱导及过氧化物同工酶的测定  

Callus induction on Aesculus chinensis and the determination of peroxidase isozyme

作　　者：李鹏丽[1] 穆红梅[1] 时明芝[1,2] 

机构地区：[1]聊城大学农学院,山东聊城252059 [2]山东财政学院

出　　处：《林业科技开发》2010年第4期38-41,共4页China Forestry Science and Technology

基　　金：山东省教育厅科技攻关资助项目(J07YG08)

摘　　要：以七叶树幼嫩叶片为外植体,利用正交试验研究了植物生长调节剂、碳源以及灭菌时间对七叶树叶片愈伤组织诱导的影响,并采用聚丙烯酰胺垂直凝胶电泳法对愈伤组织中过氧化物同工酶进行了测定。试验表明:作为碳源的葡萄糖与蔗糖、麦芽糖相比,更有利于诱导七叶树幼嫩叶片形成愈伤组织,0.1%HgCl2浸泡外植体10 min,起到了较理想的灭菌效果,2.4-D 2.0 mg/L与6-BA 0.4 mg/L为诱导愈伤的最佳浓度组合,测定的愈伤组织过氧化物同工酶谱带与接种前叶片的相比明显增多且不同处理间的条带也不尽相同。Plant growth regulators,carbon sources and sterilization time were studied on the callus induction from Aesculus chinensis young leaves as explant by orthogonal experimental.At the same time vertical polyacrylamide gel using electricity beach on POD calli was determined.The result showed that glucose was more conducive to the formation of leaf-induced calli from Aesculus chinensis than sucrose and maltose as a carbon source.The appropriate sterilization method was 0.1% HgCl2 soaking explants for 10 min.The optimal concentrations for callus induction was 2.4-D 2.0 mg/L and 6-BA 0.4 mg/L.Peroxidase isozyme bands increased significantly compared with the pre-inoculation of leaves and the difference in treatment between the different bands.

关 键 词：七叶树 诱导 愈伤组织 同工酶测定 

分 类 号：S567.239[农业科学—中草药栽培]