蔷薇属植物远缘杂交组合的幼胚拯救  

Research on the immature embryo rescue in Rosa distant hybridization

作　　者：金菁[1] 王国良[2] 刘冠楠[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业委员会

出　　处：《林业科技开发》2010年第4期72-76,共5页China Forestry Science and Technology

基　　金：国家"十一五"科技支撑项目"优质高产月季新品种选育(编号:2006BAD01A1805)";江苏省科技支撑项目"高抗浓香型大花藤本月季新品种选育"(编号:BE2008400)

摘　　要：蔷薇属植物的远缘杂交是月季品种改良的主要途径,为获得蔷薇属植物远缘杂交植株,以‘白雪山’(Rosa hybrida‘white mountain’)为母本,粉红香水月季(R.odoratavar.erubescens)、‘湖中月’(R.hybrida‘huzhongyue’)、‘紫红香’(R.hybrida‘zihongxiang’)、‘香粉蝶’(R.hybrida‘xiangfendie’)为父本杂交。于杂交后15～40天每5天取幼胚离体培养,并借助石蜡切片技术观察各时段幼胚发育情况。结果表明,正常培养条件下,在MS+6-BA1.0 mg/L+NAA0.05 mg/L培养基上,‘白雪山’、‘湖中月’、‘白雪山’ב香粉蝶’、‘白雪山’ב紫红香’可直接萌发并成苗,但成苗率较低,‘白雪山’×粉红香水月季在该培养基上无法萌发。对35天‘白雪山’×粉红香水月季杂交幼胚进行(10±2)℃、光强1 600～2 000 lx预处理后,在相同培养基上可获得杂交幼胚。加入GA3后可提高各幼胚萌发率,各类型幼胚萌发的最适浓度不同。Hybridization with Rosa hybrida ＇white mountain＇ as mother parent and R.odorata var.erubescens,R.hybrida hybrida ＇huzhongyue＇,R.hybrida ＇zihongxiang＇,R.hybrida ＇xiangfendie＇ as father parent,respectively.the early embryos were cultured from 15～40 d since artificial pollination in each 5 days.Paraffin section was also used to observe morphological development of early embryo.In normal cultural environment,early embryos of R.hybrida ＇white mountain＇×R.hybrida ＇huzhongyue＇,R.hybrida ＇white mountain＇×R.hybrida ＇zihongxiang＇,R.hybrida ＇white mountain＇×R.hybrida ＇xiangfendie＇,cultured at MS＋6-BA 1.0 mg/L＋NAA 0.05 mg/L,could bourgeon at low shoot rate,while R.hybrida ＇white mountain＇×R.odorata var.erubescens couldn＇t bourgeon.After the pretreatment with（10±2）℃ and 1 600～2 000 lx,early embryos of R.hybrida ＇white mountain＇×R.odorata var.erubescens bourgeoned at same media.Media added with GA3 would increase the germinate rate of all kinds of early embryos,and different kinds of embryos had their own optimal concentration.

关 键 词：蔷薇属植物 幼胚拯救 杂交组合 

分 类 号：S334.3[农业科学—作物遗传育种]