白蜡树组织培养快繁体系的建立  

Establishment of mass propagation system for Fraxinus chinensis

作　　者：毕君[1,2] 王春荣[1,2] 曹福亮[3] 

机构地区：[1]河北省林木良种工程技术研究中心,石家庄050061 [2]河北省林业科学研究院 [3]南京林业大学

出　　处：《林业科技开发》2010年第4期79-82,共4页China Forestry Science and Technology

基　　金：国家"十一五"科技支撑计划(编号:2006BAD03A01)

摘　　要：选用白蜡树带腋芽半木质化茎段为外植体,进行组织培养技术研究。结果表明:适宜的消毒处理为体积百分数75%酒精15 s+0.1%(体积百分数)HgCl23 min;启动培养基为1/2MS+6-BA1.0 mg/L+IBA 0.1(或NAA0.05)mg/L;最佳增殖培养基为MS+6-BA3.0 mg/L+IBA 0.2 mg/L;根诱导以1/2MS+NAA1.0 mg/L、1/2MS+IAA0.5 mg/L、1/2MS+NAA0.2 mg/L+IAA0.5 mg/L为宜,生根率达100%。Take the stem-segment with axillary buds of Fraxinus chinensis as explants in tissue culture.The results showed that the best disinfectant was dip in 75% ethyl alcohol for 15 s,then agitating in a solution of 0.1% HgCl2 for 3 min;Best initial medium was 1/2 MS＋6-BA 1.0 mg/L＋IBA 0.1 or NAA 0.05 mg/L;The best multiplication medium was MS＋6-BA 3.0 mg/L＋IBA 0.2 mg/L;The best induction medium for roots was 1/2 MS＋NAA 1.0 mg/L or 1/2 MS＋IAA 0.5 mg/L or 1/2 MS＋NAA 0.2 mg/L＋IAA 0.5 mg/L,the rooting rate was 100%.

关 键 词：白蜡树 茎段 组织培养 快繁 

分 类 号：S68[农业科学—观赏园艺]