曼地亚红豆杉组织培养  

Tissue culture of Taxus media

作　　者：翟合欢[1] 傅玉兰[1] 

机构地区：[1]安徽农业大学林学与园林学院,合肥230036

出　　处：《林业科技开发》2010年第4期98-100,共3页China Forestry Science and Technology

基　　金：国家"十一五"科技支撑计划(编号:2006BAD03A16)

摘　　要：为了进一步解决具有抗癌活性的紫杉醇资源短缺的问题,以红豆杉的幼嫩茎段为外植体筛选出最佳灭菌措施及继代增殖中6-BA、NAA、TDZ不同激素的最佳配比。结论如后:70%(体积百分比,后同)酒精浸泡20 s+0.3%(体积百分数,后同)升汞浸泡6 min灭菌效果最好;最适宜腋芽继代增殖的培养基配方为MS+6-BA 0.15mg/L+NAA1.0 mg/L+TDZ 0.01 mg/L+食糖30 g/L+琼脂8 g/L+AC 0.4 g/L,pH值为5.8。In order to solve the problem of the resources shortage for taxol of anti-cancer activity,based on the experiment of explants from the tender stem segments of Taxus media to choose the optimum technique of sterilization and the best proportion of the subculture with 6-BA,NAA,TDZ different hormones,got results as follows： the optimum technique of explant sterilization of Taxus media was in 70% ethanol for 20 s and 0.3% HgCl2 for 6 min;the most appropriate medium for multiplication axillary bud subculture was MS＋6-BA 0.15 mg/L＋NAA 1.0 mg/L＋TDZ 0.01 mg/L＋sugar 30 g/L＋Agar 8 g/L＋AC 0.4 g/L,pH 5.8.

关 键 词：曼地亚红豆杉 紫杉醇 继代增殖 

分 类 号：S791.49[农业科学—林木遗传育种]