高效液相色谱法同时测定旱芹中东莨菪内酯和滨蒿内酯含量  

Simultaneous determination of scopoletin and scoparone in Apium graveolens L.by HPLC

作　　者：谭亮[1] 张琦[2] 范旭航[2] 陈晓辉[1] 钱忠直[1,3] 毕开顺[1] 贾英[2] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]沈阳药科大学中药学院,沈阳110016 [3]国家药典委员会,北京100061

出　　处：《中南药学》2010年第7期503-506,共4页Central South Pharmacy

摘　　要：目的建立同时测定旱芹中东莨菪内酯和滨蒿内酯含量的高效液相色谱法,并考察旱芹中这2种成分的含量与产地、采收期的关系,为旱芹药材的质量控制提供依据。方法色谱柱:Diamonsil C18柱(250mm×4.6mm,5μm);流动相:甲醇-水(32∶68,v/v),流速:1.0mL.min-1;检测波长:340nm,柱温:35℃。结果东莨菪内酯和滨蒿内酯的浓度分别在0.510 0～10.20μg.mL-1(r=0.999 4)、0.537 5～10.75μg.mL-1(r=0.999 2)与峰面积呈良好的线性关系(n=6),平均回收率(n=9)分别为95.1%、96.4%。结论该方法操作简便,结果准确,重复性好,可作为旱芹中香豆素类成分的质量控制方法。Objective To establish an HPLC method for the simultaneous determination of scopoletin and scoparone in Apium graveolens L.A relationships between the contents of the two compounds,and their origins and collection pe-riod were considered.Methods The Diamonsil C18（250mm × 4.6mm,5μm）column was used.The mobile phase consisted of methanol-water（32∶68,v/v）at 1.0mL.min-1,the detection wavelength was 340nm and the column temperature was 35℃.Results The method had good linearity within 0.510 0-10.20μg.mL-1 for scopo-letin（r=0.999 4）;0.537 5-10.75μg.mL-1 for scoparone（r=0.999 2）,respectively.The average recovery（n =9）was 95.1%for scopoletin and 96.4%for scoparone.Conclusion The method is simple,accurate and reproduci-ble.It can be used for the quality control of coumarins ingredient in Apium graveolens L.

关 键 词：东莨菪内酯 滨蒿内酯 旱芹 香豆素类成分 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1