影响根癌农杆菌转化水稻频率的因素研究  

Study on the Influence Factors of the Frequemcy of Agrobacterium mediated Transformation in Rice ( Oryza sativa L.)

作　　者：王力[1] 张云孙[1] 陈屹 高照 李静 蔺兆星 

机构地区：[1]云南大学生物系

出　　处：《云南大学学报（自然科学版）》1999年第2期116-119,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省自然科学基金

摘　　要：根癌农杆菌与来自水稻成熟种子的愈伤组织共培养，将外源基因导入水稻愈伤组织，并获得了转基因植株．通过比较影响根癌农杆菌转化频率的各种因素，表明在转化过程中酚类化合物和单糖的加入使农杆菌的转化频率提高了０．９％～１７．４％．We successfully introduced foreign genes to calli of rice and got transgenic plants by Agrobacterium tumefaciens cocultured with calli induced from mature seeds of rice.Affer investigating the various factors influencing the transformation frequency of Agrobacterium tumefaciens ,the results indicated that transformation frequency was increased by 0.9% 17.4% supplemented with phenolic compounds and monosaccharide during trans formation step.The dilution way of Agrobacterium tumefaciens played an important role influencing the transformation frequency of Agrobacterium tumefaciens .

关 键 词：水稻 愈伤组织 根癌农杆菌 转化频率 影响因子 

分 类 号：S511.035.3[农业科学—作物学]