两株双价痢疾菌苗免疫小鼠后GALT中CD4^+、CD8^+ T细胞亚群的反应  

Changes of CD4+ and CD8 + T subsets in GALT of mice after immunization with two different bivalent Shigella vaccines

作　　者：石辛甫[1] 高杰英[1] 彭红[1] 邢丽[1] 刘育红[1] 陈志华[1] 曾关富[1] 

机构地区：[1]军事医学科学院微生物流行病研究所免疫室

出　　处：《中华微生物学和免疫学杂志》1999年第1期29-32,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家863计划经费资助

摘　　要：目的ＦＳＭ－２１１７和ＦＳ－５４１６是两株具有不同生物表型的福氏、宋内氏双价痢疾菌苗株，ＦＳＭ－２１１７无侵袭表型，无溶血活性，不表达侵袭蛋白抗原（Ｉｐａ－），ＦＳ－５４１６则为Ｉｐａ＋，本实验是为了观测两株菌苗（Ｉｐａ－，Ｉｐａ＋）免疫后引起的肠粘膜相关淋巴组织（ＧＡＬＴ）中的细胞免疫反应，以探明痢疾菌苗在肠粘膜的免疫保护机制。方法以ＢＡＬＢ／ｃ小鼠随机分成两组，每组２５只，灌胃免疫ＦＳＭ－２１１７及ＦＳ－５４１６，８×１０８ＣＦＵ／只，分别在１，４，７，１０，１３天随机取各组小鼠５只分离ＧＡＬＴ淋巴细胞；同时设一组ＰＢＳ对照组，以间接免疫荧光法检测ＧＡＬＴ中诱导部位〔派伊尔小结（ＰＰ）、肠系膜淋巴结（ＭＬＮ）〕及效应部位〔上皮间淋巴细胞（ＩＥＬ）、粘膜固有层（ＬＰＬ）〕Ｔ细胞亚群的变化，荧光显微镜计数２００个细胞计算阳性率。结果用ＳＡＳ软件，单因素方差分析，表明两株双价菌苗株均可引起肠粘膜ＧＡＬＴ中ＣＤ４＋、ＣＤ８＋淋巴细胞亚群改变，不同粘膜部位免疫反应不同。诱导部位（派氏小结、肠系膜淋巴结）ＣＤ４＋细胞亚群明显升高，末次免疫后第７天达峰值，而后迅速下降；在效应部位，粘膜固有层表现为ＣＤ４＋细胞亚群明显?Objectives FSM 2117 and FS 5416 are two bivalent hybrid strains of S. flexneri and S. sonnei, constructed by our laboratory. The differences of them are that the FS 54116 expresses four invasive plasimid antigens and has a good contact heamolysis activity(CHA +), but the FSM 2117 does not. To observe the mechanism of immune protection of Shigella vaccine, the changes of CD4 + and CD8 + T subsets in GALT in mice are detected. Methods BALB/c mice were divided into two groups, 25 mice per group. The two groups were immunized respectively with the two vaccines(8×10 8CFU/per mouse) four times with an interval of seven days by pouring the vaccine down the throat. The Peyer's patch(PP), mesenteric lymph nodes(MLN), intraepithelial(IE), lamina propria(LPL) and spleen(SP) lymphocytes were isolated of the same time on the first, fourth, seventh, tenth, thirteenth day at random after immunization. A PBS immunized group was detected as control. An indirect immuno fluorescence assay was done. And 200 cells were counted under fluorescence microscope. Results The data were analysed with SAS software (ANOVA). And it could be observed that the CD4 + subset of the two immunized groups showed a significant rise in PP and MLN of induced sites and LP effective site, and the CD8 + subset rose in IE of effective sites. The responses of both the two groups were significantly different from that of controls(FSM 2117, P ≤0 01,FS 5416 P <0 01). Conclusions Immunization with the two Shigella vaccines can result in immune responses in GALT in mice, and both two vaccines have better immunogenicity.

关 键 词：痢疾菌苗 肠粘膜 淋巴组织 T淋巴细胞亚群 

分 类 号：R378.25[医药卫生—病原生物学]