藿香挥发油 黄柏生物碱对高温刺激下IEC-6细胞分泌SOD GSH-Px和MDA的影响  

Effects of Herba Agastachis essential oil and Cortex Phellodendri alkaloid on the antioxidation of IEC-6 in high-temperature

作　　者：王伟[1] 徐淑芳[2] 郭凯军[1] 许剑琴[3] 程桂林[1] 余进[1] 王霞泰[1] 王安如[3] 刘凤华[1] 

机构地区：[1]北京农学院动物科技系,北京昌平102206 [2]北京市农林科学院畜牧兽医研究所,北京海淀100097 [3]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2010年第7期60-64,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30771591;30771566);国家"十一五"科技支撑计划(2008BADB4801;2008BADPAB07;2006BAD31B05-2);北京市自然科学基金(6082007)

摘　　要：探讨不同浓度的藿香挥发油,黄柏生物碱对高温刺激下IEC-6细胞SOD、GSH-Px和MDA的影响。建立IEC-6细胞株的高温刺激模型,添加适宜浓度的藿香挥发油和黄柏生物碱.检测上述指标的变化。结果表明,高温刺激能显著降低IEC-6细胞增殖(P<0.05);200g/mL的藿香挥发油和100g/mL的黄柏生物碱都能使高温刺激下IEC-6细胞SOD、GSH-Px水平极显著(P<0.01)升高和降低;MDA含量极显著(P<0.01)降低。结论:藿香挥发油和黄柏生物碱能显著改善高温刺激诱导的IEC-6细胞增殖、SOD、GSH-Px和MDA分泌的变化,提高细胞抗氧化能力。The purpose of this study was to investigate the effects of Herba Agastachis essential oil and Cortex Phellodendri alkaloid on SOD,GSH-Px and MDA contents of IEC-6 cells after high-tempera- ture treatment. This study consists of establishing the high-temperature-induced IEC-6 model,adding ap- propriate concentration of the essential oil and alkaloid and then assaying the contents of above-mentioned detection index. Results showed that high temperature treatment can significantly inhibit IEC-6 cell prolif- eration （P0. 05）; both of 200 /μg/mL essential oil and 100 g/mL alkaloid conspicuously （P0. 01） in- crease the levels of SOD and GSH-Px and decrease the content of MDA on IEC-6 cells treated by high tem- perature. Herba Agastachis essential oil and Cortex Phellodendri alkaloid can improve the changes of GSH-Px,SOD and MDA of IEC-6 cells induced by high temperature and enhance the antioxidant function of cells.

关 键 词：IEC-6 藿香挥发油 黄柏生物碱 MDA SOD GSH-PX 

分 类 号：S853.74[农业科学—临床兽医学]