紫外分光光度法测定赤芍中芍药甙的含量  

Determination of paeoniflorin in Radix Paeoniae Rubra and radix by UV spectrophotometry

作　　者：郑湘娟[1] 余淑娴[2] 李玉琳[2] 

机构地区：[1]南昌大学基础化学实验中心,南昌330031 [2]南昌大学理学院化学系,南昌330031

出　　处：《食品科技》2010年第7期262-263,共2页Food Science and Technology

基　　金：江西省教育厅科技计划项目(GJJ09044);南昌大学校基金项目(Z04293)

摘　　要：目的:建立赤芍提取物中芍药甙含量的测定方法。方法:采用紫外分光光度法,检测波长为228nm。结果:检测浓度在0.01～0.2mg/mL范围内与吸收度线性关系良好(R=0.98352)。结论:方法简便、快速、准确,样品提取后不经分离可直接稀释测定。Objective：To establish a method for the content determination of paeoniflorin in Radix Paeoniae Rubra.Methods：UV Spectrophotometry was adopted in the determination with the detection wavelength set at 228 nm.Results：Good linear relationship with the absorbability was achieved when the detection concent ration of eldrin was within arange of 0.01～0.2 mg/mL（R=0.98352）.Conclusion：This method was simple,rapid and accurate.

关 键 词：赤芍 芍药甙 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]