两种新型杀虫剂对蚯蚓精子形态的影响  

EFFECT OF TWO NEW PESTICIDES ON SPERM MORPHOLOGY OF EARTHWORMS (EISENIA FOETIDA)

作　　者：孔志明[1,2] 臧宇[1,2] 沈进萍 罗屿[1,2] 钟远 

机构地区：[1]南京大学污染控制与资源化研究国家重点实验室 [2]南京大学环境科学与工程系

出　　处：《癌变．畸变．突变》1999年第1期14-16,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目,项目编号39670153

摘　　要：本文提出了一种检测及评价土壤生态系统中遗传毒性的新方法。赤子爱胜蚓（Ｅｉｓｅｎｉａｆｏｅｔｉｄａ）经人工土壤法染毒，暴露于不同剂量的两种新型杀虫剂———呲虫啉和抑食肼中，观察它们对蚯蚓精子形态的影响。实验结果表明，呲虫啉在浓度大于０．５ｍｇ／ｋｇ干土时对蚯蚓精子畸变率有明显影响（Ｐ＜０．０１），并呈现剂量效应关系；而抑食肼在浓度高达１００ｍｇ／ｋｇ干土时，与对照组相比才有显著差异（Ｐ＜０．０５）。In this paper, a new method to assess the environmental safety of pesticide has been proposed in terms of sperm deformity of the earthworm (Eisenia foetida). The results showed that the main sperm morphological deformity appeared to be shape changes of the sperm heads. Significant induction of sperm deformity (P<0.01) and dose effect relationship displayed when the earthworms were exposed to imidacloprid at the concentrations of more than 0.5mg/kg dry soil. However, The sperm deformity frequency of RH-5849-exposed groups did not show significant difference (P<0.05) from the negative control until the dose reached 100 mg/kg dry soil. This study suggests that the detection of sperm abnormality in earthworms could provide an early warning system of polluted environment.

关 键 词：吡虫啉 抑食肼 赤子爱胜蚓 精子形态 杀虫剂 

分 类 号：TQ450.26[化学工程—农药化工]