促红细胞生成素对脑室周围白质软化新生大鼠髓鞘磷脂蛋白和神经生长相关蛋白的影响  

Effects of Erythropoietin on Myelin Basic Protein and Growth-Associated Protein-43 in Neonatal Rats with Periventricular Leukomalacia

作　　者：胡燕[1] 蒋犁[1] 张敏[1] 王婷[2] 何农跃[2] 

机构地区：[1]东南大学附属中大医院产科,南京210009 [2]东南大学生物电子学国家重点实验室,南京210096

出　　处：《实用儿科临床杂志》2010年第14期1046-1048,共3页Journal of Applied Clinical Pediatrics

基　　金：生物电子学国家重点实验室开放研究基金(6807039002)

摘　　要：目的探讨重组人促红细胞生成素(rEPO)对脑室周围白质软化(PVL)新生大鼠的神经保护作用。方法新生5 d(P5)SD大鼠96只随机分成3-硝基丙酸(3-NPA)脑内注射损伤组(3-NPA组)、rEPO治疗组(rEPO组)和假手术对照组(PBS组),其中rEPO组于脑损伤前0.5 h予rEPO腹腔注射(50 U/10 g体质量),3组于术后不同时间点行HE染色和免疫荧光标记,计算髓鞘磷脂蛋白(MBP)和神经生长相关蛋白(GAP-43)阳性细胞数,术后24 d(P29)行神经行为学测定。结果 HE染色显示3-NPA组P7时皮质下及脑室周围白质出现不同程度疏松及液化灶,P14出现脑室扩大;P7时,PBS组和rEPO组注射部位周围脑组织疏松液化,P14、P12时未见明显脑室扩大。P7、P8时,3组脑室周围白质中均有MBP阳性细胞表达,P14、P30时,3-NPA组阳性细胞数低于PBS组和rEPO组(Pa<0.05);P30时,3-NPA组GAP-43阳性细胞较rEPO组和PBS组减少(Pa<0.05);神经行为学测定显示3-NPA组较PBS组和rEPO组反应差(P<0.05)。结论 rEPO可减轻新生大鼠脑白质损伤、促进神经生长修复,发挥远期神经保护作用。Objective To investigate the protective effects of recombinant human erythropoietin （rEPO） on neuron of the neonatal rats with periventricular leukomalacia （PVL） induced by intracerebral injection of 3 - nitropropionic acid （3 - NPA）. Methods Ninety - six Sprague- Dawley （SD） rats 5 days post- neonatal （P5） were divided into brain injury group （3 -NPA group）, rEPO treated group （rEPO group） and sham operated control group（PBS group）, rEPO group：rEPO（50 U/10 g） was intraperitoneal injected at half an hour before in- jecting 3 - NPA. HE staining and immunofluorescence methods were used to detect the brain change and expression of myelin basic protein （MBP） and growth -associated protein -43 （GAP -43 ）, and the positive cells at different time point post - operation were detected. Neu- robehavioral tests were performed on the rats 24 days post - operation（P29）. Results HE staining showed that tissue necrosis was observed in the periventricular white matter in 3 - NPA group at P7 ,and left ventricular dilation and decrease of the corpus callosum area at P14. The injection site of brain tissue was loose in PBS group and rEPO group at P7, and no ventricular dilation at P14. MBP immunoreactive cells were observed in the 3 groups at P7 and PS. MBP sign cells were decreased in 3 - NPA group compared with PBS group and rEPO group at P14 and P30（Pa 〈0.05）. GAP -43 positive cells in 3 - NPA group were less than those of PBS group and rEPO group at P30（Pa 〈0.05）. In the neurobehavioral tests, the achievement in the rEPO group and PBS group were clearly higher than that of 3 - NPA group（ Pa 〈 0. 05 ）. Conclusions EPO treatment can significantly reduce brain white matter damage and promote nerve growth and repair, improve neurodevelopmental outcome and operate the long - dated neuroprotective effeets in PVL.

关 键 词：促红细胞生成素 脑室周围白质软化 髓鞘磷脂蛋白 神经生长相关蛋白 大鼠 新生 

分 类 号：R722.1[医药卫生—儿科]