左旋多巴固体脂质纳米粒的制备及包封率测定  

Preparation and entrapment efficiency determination of solid lipid nanoparticles loaded levodopa

作　　者：詹水明[1] 侯冬枝[1] 平其能[2] 徐岩[3] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]中国药科大学药学院,江苏南京210009 [3]华中科技大学附属协和医院,湖北武汉430070

出　　处：《中国医院药学杂志》2010年第14期1171-1175,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:30700881);广东药学院人才引进科研启动基金项目(编号:2007YKX20)

摘　　要：目的:用微乳法制备左旋多巴固体脂质纳米粒(LDP-SLN),并建立包封率的测定方法。方法:通过绘制三元相图,采用微乳法制备LDP-SLN,用TEM和激光粒度仪进行了颗粒形貌和粒径分布的研究,用葡聚糖凝胶层析法分离测定包封率。对其粒径、形态、包封率等理化性质进行研究,并考察其稳定性。结果:实验制得LDP-SLN为稳定的略泛蓝色乳光的纳米混悬液,在透射电镜下显示为较为均匀的球体,激光粒度测定平均粒径为108nm,多分散系数1.153;4℃放置2个月,粒径、包封率无显著变化。包封率测定的线性范围为2～100mg·mL-1,线性良好(r=0.9999),精密度符合要求,LDP-SLN上柱洗脱后分离度和回收率均符合要求。结论:该研究中制备了物理性质较为稳定的LDP-SLN,建立了合适的包封率测定方法,并考查初步稳定性较好。OBJECTIVE Levodopa solid lipid nano particles （LDP-SLN） was prepared ,and an efficient method for determi nation of entrapment efficiency（ EE% ）was established. METHODS LDP-SLN was prepared through the micro emulsion tech nology prepared by drawing ternary phase diagram, The morphology was examined by transmission electron microscope, the particle size was determined by laser granularity equipment, and the entrapment efficiency（EE%） was determined with the Sephadex gel chromatogram. The mean particle size,drug entrapment efficiency （EE%） and long-term physical stability of the SLN were investigated. RESULTS The loaded SLN dispersion was a stable milk like suspension,and was uniform spheres un der the electron microscope,the mean particle size was 108 nm,muhi factor spread was 1. 153. There were no obvious changes of the particle size and entrapment efficiency at 4 ℃ within two month. The method for determination of entrapment efficiency showed a good linearity range from 2 to100 mg·L^-1 （r= 0. 9999） ,the precision met the requirements. SLN on drug eluting after the separation and recovery met the requirements. CONCLUSION The LDP-SLN prepared by micro-emulsion technology has good stability, the method for determination of entrapment efficiency established is applicable to the system entrapmcnt effi ciency of SLN. The physical stability was relative stable.

关 键 词：左旋多巴 固体脂质纳米粒 包封率 

分 类 号：R943[医药卫生—药剂学]