动态浊度法比较不同方法对内毒素的清除效率  

Comparison on the effect of different methods of depyrogenation by kinetic turbidimetric assay

作　　者：李友[1] 龚慕辛[1] 关怀[1] 穆阳[1] 马莉[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中国医院药学杂志》2010年第14期1175-1177,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30701091);北京市优秀人才培养资助项目(编号:20071D0501800248)

摘　　要：目的:对热压灭菌、活性炭吸附、微孔滤膜、超滤和微波等几种除内毒素方法进行比较。方法:向无内毒素细菌内毒素检查用水外加定量内毒素,采用动态浊度法测定除内毒素前后内毒素的含量,考察几种方法的除内毒素效率。结果:活性炭吸附法效果最好,对无内毒素溶剂内毒素的清除率均在99.0%以上。结论:活性炭吸附和超滤法对内毒素有很好的清除效果。动态浊度法是内毒素定量检测的一种简便、快捷的方法。OBJECTIVE To compare the effect of depyrogenation of heat pressure sterilization,activated carbon adsorption, micropore filter method,uhrafihration and microwave. METHODS Kinetic turbidimetric assay were used to inspect the effeet of the 5 methods of depyrogenation by adding the extra endotoxin to the nonpyrogenic bacterial endotoxin test water. RESULTS Activated carbon adsorption had the best effect on depyrogenation and the clearance rate was above 99. 0%. CONCLUSION Activated carbon adsorption and uhrafihration had good effect on depyrogenation; kinetic turhidimetric assay was a fast and convenient method in endotoxin quantitation test.

关 键 词：动态浊度法 内毒素 活性炭吸附 超滤 

分 类 号：R927.1[医药卫生—药学]