TiO2薄膜表面构建催化活性层改善抗血小板黏附性能  

Building a catalytic activity layer on TiO_2 film surface for improving its anti-platelet adhesion ability

作　　者：张利萍[1] 翁亚军[1] 周玉娟[1] 黄楠[1] 陈俊英[1] 李遂焰[2] 

机构地区：[1]西南交通大学材料科学与工程学院,材料先进技术教育部重点实验室,四川成都610031 [2]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《功能材料》2010年第7期1158-1161,共4页Journal of Functional Materials

基　　金：国家重点基础研究发展计划(973计划)资助项目(2005CB623904);博士后基金资助项目(20090450171);国家自然科学基金资助项目(30900295)

摘　　要：一氧化氮(nitric oxide,NO)是心血管系统的信号分子,具有抗凝血抗增生多种生物学功能。在TiO2薄膜表面固定生物分子胱胺、硒代胱胺,构建催化活性层,催化供体释放NO,从而使改性后的材料表面具有抗凝效应。构建分为两个步骤,首先通过膦酸单分子层自组装固定十二烷基膦酸,再借助光化学方法在材料表面固定4-叠氮基苯甲酸分子使表面羧基官能团化;随后,用EDC/NHS/MES体系活化表面羧基,进而固定胱胺、硒代胱胺。傅里叶红外吸收光谱(Fourier transform infrared spectroscopy,FTIR)与X光电子能谱(X-ray photoelectron spectroscopy,XPS)结果显示各分子均在TiO2薄膜表面成功固定。体外催化实验结果证实了催化活性层的催化活性。该改性层可原位催化内源性一氧化氮供体释放出NO,改善材料表面抗血小板粘附性能,其在体外血小板黏附实验中得到证实。改善了TiO2薄膜的生物相容性,为无机材料的生物活化提供了一种新途径。Nitric oxide （NO）,with a variety of biological properties and functionalities such as anti-coagulant and anti-proliferation of cells,is an important signal molecule in the cardiovascular system.In this work,for improving the anti-platelet adhesion ability of the TiO2 film,a catalytic activity layer was built by grafting the cysteamine or cysteamine selenocysteine onto the TiO2 film.Firstly,the dodecyl phosphonic acid was self-assembled onto the TiO2 film surface with aim to gain the alkyl groups,the 4-azido acid molecules was then bonded to the dodecyl phosphonic acid by photochemical methods for forming a functional layer with carboxyl groups.Furthermore,the cysteamine or cysteamine selenocysteine was chemically connected onto the above surface due to the carboxyl group activated by the EDC/NHS/MES solution system.The FTIR absorption spectroscopy （FTIR） and the X-ray photoelectron spectroscopy （XPS） results showed that,the molecule of cysteamine and cysteamine selenocysteine was successfully grafted to the TiO2 film surface,respectively.The in vitro catalytic experiment results confirmed that the modified layer possessed the catalytic activity.The platelet adhesion experiment disclosed that the modified TiO2 film surface could prohibit the adhesion behavior of platelets.This work successfully improved the biocompatibility of the TiO2 film,and provided a novel method for the bio-catalytic activation of the inorganic materials.

关 键 词：TIO2薄膜 光化学 催化活性 一氧化氮 血小板 

分 类 号：R318.08[医药卫生—生物医学工程]