CaM拮抗剂W7对PEG胁迫下番茄幼苗根系生长和ABA、多胺动态变化的影响  

Effect of calmodulin antagonist W7 on dynamic changes in endogenous ABA,polyamine contents and growth of tomato seedling roots under PEG stress

作　　者：张春梅[1] 邹志荣[2] 黄志[2] 

机构地区：[1]河西学院农学系,甘肃张掖734000 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2010年第4期64-68,共5页Agricultural Research in the Arid Areas

基　　金：国家"十一五"攻关项目(2007BAD79B04)

摘　　要：以耐旱性不同的两个番茄品种为试材,采用营养液水培,研究了CaM拮抗剂W7〔N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide〕预处理对聚乙二醇(PEG-6000)模拟干旱胁迫下番茄幼苗根系生长及内源ABA、游离态多胺含量的影响。结果表明,用200 mmol/L W7预处理24 h,然后进行干旱胁迫处理,番茄根系内源ABA和多胺含量低于未经W7预处理的PEG胁迫处理下的值,W7处理削弱了番茄对胁迫逆境的抵抗能力,幼苗生长变弱,表明Ca2+-CaM信使系统通过影响多胺的含量参与调节干旱胁迫下多胺的代谢过程,对抵抗干旱逆境胁迫有积极作用。两个品种相比较,‘毛粉802’在PEG胁迫下可以积累更多的亚精胺(Spd)和精胺(Spm),受W7的影响小于‘皇冠’,表现出较强的耐旱性。The effect of calmodulin antagonist W7 on the changes in endogenous ABA,polyamine contents and growth of tomato seedling roots under hypoxia stress was studied.The results showed that after pre-treatment with 200 mmol/L W7 for 24 h before the hypoxia stress,the contents of free spermine and free spermidine were lower than those in tomato seedling roots by only hypoxia stress treatment,which suggested that the pretreatment with W7 could lead to the decrease in polyamine contents in tomato roots compared to the only treatment of hypoxia stress,reduce the ability of cucumber against hypoxia stress,and make the plant growth become weaker.Finally,it was indicated that the effect of W7 was found smaller on ＇Maofen802＇ than on ＇Huangguan＇ cultivar of tomato,and ＇Maofen802＇ had greater capability against PEG stress.

关 键 词：CaM拮抗剂W7 PEG胁迫 番茄 脱落酸 多胺 

分 类 号：S641.2[农业科学—蔬菜学]