病斑、病斑附近烟叶和正常烟叶的FTIR研究  

FTIR Study on the Leaf Spots,Near-Spot and Normal Tobacco Leaves

作　　者：任先培[1] 刘刚[1] 周在进[1] 刘飞[1] 李志永[1] 宋黎立 

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明650092 [2]文山州烟草公司文山县分公司,云南文山663000

出　　处：《光谱学与光谱分析》2010年第8期2120-2123,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30960179;10764006);云南省自然科学基金项目(2007A048M)资助

摘　　要：用傅里叶变换红外光谱法研究了赤星病、角斑病和气候型斑点病3组病斑、病斑附近烟叶和正常烟叶,发现3组烟叶样品红外光谱的吸光度比A1631/A1025呈现相同的变化趋势,有正常烟叶<病斑附近烟叶<病斑的规律。对3组烟叶样品整个光谱区的原始光谱和二阶导数光谱进行距离分析,结果显示,病斑附近烟叶与正常烟叶的Pearson相关系数大于病斑与正常烟叶的Pearson相关系数,即与病斑相比,病斑附近烟叶与正常烟叶具有更紧密的关系。吸光度比A1631/A1025和Pearson相关系数的这种变化趋势表明病斑附近烟叶的化学成分在逐渐改变,即病斑附近烟叶处于正常和病变的过渡状态。研究结果表明,傅里叶变换红外光谱有望成为诊断烟草潜育期病害的方法。Fourier transform infrared spectroscopy （FTIR） was used to study three group tobacco leaves of brown spot,angular spot and weather speck,with each group being composed of three samples,namely,leaf spots,near-spot and normal tobacco leaves.The results indicate that the Absorption ratio A1 631/A1 025 of the three group tobacco leaves showed the same change tendency,with the normal tobacco leavesthe near-spot leavesthe leaf spots.For a more objective and comprehensive analysis,the original and second-derivative spectra were selected for distance analysis in the whole region.The results show that the Pearson correlation coefficient of the near-spot leaves and normal leaves is greater than the corresponding coefficient of leaf spots and normal leaves,which suggest that the near-spot leaves and normal leaves have a closer relationship compared with the leaf spots and normal leaves.The ratios of the A1 631/A1 025 and Pearson correlation coefficients show that the chemical composition of the near-spot leaves changed gradually,that is,the near-spot leaves were in a transient state between normal and disease leaves.In conclusion,FTIR spectroscopy is a promising technique for diagnosing tobacco disease in the incubation period.

关 键 词：傅里叶变换红外光谱 烟草病害 诊断 距离分析 

分 类 号：O657.3[理学—分析化学]