75份腰果种质资源的RAPD分析  

RAPD Analysis of 75 Accessions of Cashew Germplasm(Anacardium occidentale L.)

作　　者：黄伟坚[1] 黄海杰[1] 张中润[1] 王金辉[1] 吴家和[2] 梁李宏[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所农业部热带作物种质资源利用重点开放实验室,海南儋州571737 [2]中国科学院微生物研究所植物基因组学国家重点实验室,北京100101

出　　处：《热带作物学报》2010年第6期894-901,共8页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项资助项目(No.PZS059);中国热带农业科学院重点科技基金资助项目(No.Rky0401)资助

摘　　要：利用RAPD技术,对75份国内现有的腰果种质资源进行遗传亲缘关系及分类研究。利用45个随机引物对75份腰果种质资源的基因组DNA进行PCR扩增,共扩增出407条谱带,其中多态性谱带为294条,多态率达72.24%,表明75份腰果种质资源具有丰富的多态性。75份国内现有的腰果种质遗传相似性分析结果表明,各种质资源间的遗传相似系数在0.690~0.936之间,平均相似系数为0.841;通过UPGMA法建立了75份腰果种质资源的亲缘关系聚类图,当D=0.79时,将75份腰果种质划分成16个类群。分析结果表明多数来源相同的种质资源表现出较为密切的亲缘关系。Seventy-five accessions of Anacardium occidentale L.collected in China were analyzed by random amplified polymorphic DNA（RAPD） markers to evaluate their genetic variability and relationships.Fortyfive random primers were used for the amplification of random DNA sequences and generated 407 bands including 294 polymorphic band with 72.24% polymorphism,which revealed a high degree of genetic diversity among the 75 collected accessions.The genetic similarity analysis demonstrated that their genetic similarity coefficients were between 0.690 and 0.936 and the mean was 0.841.The phylogenetic dendro gram with UPGMA revealed the 75 cashew accessions could be divided into 16 clusters（D=0.79）.The results suggested that the majority of germplasm resources coming from the same ancestor presented closer relationship in phylogenesis.

关 键 词：腰果 遗传多样性 随机扩增多态性DNA 亲缘关系 

分 类 号：S667.9[农业科学—果树学]