橡胶树多主棒孢病菌毒素的纯化  

Purification and Detection of the Toxin from Corynespora cassiicola

作　　者：林春花[1] 彭建华[1] 时涛[1] 刘先宝[1] 蔡吉苗[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《热带作物学报》2010年第6期984-988,共5页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划项目(No.2007BAD48B04);中国热带农业科学院环境与植物保护研究所中央级公益性科研院所基本科研业务费专项(No.2009hzs1J014);海南省自然科学基金项目(No.309049,807041)资助

摘　　要：利用海南橡胶树多主棒孢培养液,通过透析、冷冻干燥浓缩、硫酸铵沉淀以及离子交换层析等方法对粗毒素进行了浓缩与提纯,获得的粗毒素通过生物测定和聚丙烯酰胺凝胶电泳验证。结果显示,该方法能获得较纯的具有活性的蛋白毒素,其在凝胶电泳中显示分子量约为14.4ku。A toxic substance was produced by Corynespora cassiicola, one of the main pathogens affecting rubber tree （Hevea brasiliensis）. Crude toxin was extracted from culture filtrate of HC-10 strain in the study. The toxin extract protocol involved dialysis, freeze-dried, ammonium sulfate precipitation, ion-exchange and gel filtration chromatography. Extracted toxin was detected by leaf puncture bioassay and polyacrylamide gel electrophoresis, and the results showed the molecular weight of the toxin protein was about 14.4 ku.

关 键 词：橡胶树 多主棒孢 毒素 分离纯化 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]