浊度法测定红霉素类抗生素制剂的抗菌活性  

Evaluation of Antimicrobial Activity of Erythromycins Preparation by Turbidimetric Method

作　　者：高燕霞[1] 姜建国[1] 韩彬[1] 

机构地区：[1]河北省药品检验所,河北石家庄050011

出　　处：《中国药业》2010年第14期40-42,共3页China Pharmaceuticals

基　　金：2006年河北省科技厅资助课题;项目编号:06276445

摘　　要：目的建立测定红霉素、琥乙红霉素及依托红霉素活性的浊度法,并对其进行考察评价。方法以金黄色葡萄球菌为试验菌,加菌量为1.0%～1.2%(V/V),(37±0.5)℃培养3～4 h后测定。结果红霉素抗生素线性浓度为0.08～0.8 IU/mL。结论该方法灵敏、快速、影响因素较少,可作为测定、评价红霉素、琥乙红霉素及依托红霉素活性的方法。Objective To establish a turbidimetric method for determining the antimicrobial activity of erythromycin and erythromycin ethylsuc-cinate and erythromycin estolate.Methods The test organism was Staphylococcus aureus and cultured with fresh medium（pH = 7.0）.Staphylococcus aureus inoculated with a 1.0%-1.2%（V/V） ratio was cultured at（37 ± 0.5）℃ for about 3-4 h.Results The linear range of erythromycin was 0.08-0.8 IU/mL.Conclusion The method is sensitive,rapid with less influencing factors,which is feasible to appraise the antimicrobial activity of erythromycin,erythromycin ethylsuccinate and erythromycin estolate.

关 键 词：抗菌活性 浊度法 红霉素 琥乙红霉素 依托红霉素 

分 类 号：R927.1[医药卫生—药学] R978.15