白藜芦醇调节Th细胞失衡与T-bet、GATA-3途径作用机制的探讨  

The regulatory mechanism of resveratrol in Th cell differentiation

作　　者：张敏[1] 金呈强[1] 王文涓[1] 刘仿[1] 

机构地区：[1]广东医学院微生物与免疫学教研室,湛江524023

出　　处：《国际免疫学杂志》2010年第4期329-331,共3页International Journal of Immunology

基　　金：广东省自然科学基金（NO：06028959）,广东湛江市科技计划项目（2009C3103019） 广东医学院青年基金（NO：XQ06017）

摘　　要：目的 探讨白藜芦醇对Jurkat T细胞T-bet/GATA-3表达的影响及其与调节Th1/Th2细胞分化作用机制之间的关系.方法 不同浓度的白藜芦醇刺激Jurkat T细胞48 h后,用ELISA法检测Th1/Th2细胞因子表达谱及用RT-PCR检测T-bet和GATA-3 mRNA表达的变化.结果 白藜芦醇抑制T-bet mRNA和GATA-3 mRNA的表达（F=6308,P〈0.05 F=3262,P〈0.05）.对Jurkat T细胞分泌细胞因子IFN-γ、IL-10的表达均起抑制作用,并具有浓度效应（F=2604,P〈0.05 F=2465,P〈0.05）.结论 白藜芦醇抑制Jurct T细胞由Th0向Th1细胞分化可能是通过降低转录因子T-bet表达水平进行调节,对Th2细胞的抑制作用则可能通过抑制转录因子GATA-3途径进行负调节,并具有浓度效应.Objective To investigate the role of resveratrol in the gene expression of T-bet/GATA-3 in Jurkat T cells,and to explore the mechanisms underlying this sensitizing effect of the change of TH cell subpopulation group. Methods The related cytokines about Thl/Th2 were detected by ELISA and T-bet and GATA-3 mRNA were detected by RT-PCR after Jurcat T cell are cultured with different doses of Resveratrol for 48 hours. Results Resveratrol inhibited the expressions of mRNA of T-bet and GATA-3 （ F = 6308, P 〈 0. 05 F = 3262,P〈 0.05） and the secretions of the related cytokines about Thl/Th2（F = 2604,P 〈0. 05 F =2465, P〈0. 05）. And these two effects are all concentration related. Conclusion Resveratrol could inhibit Th0 cells to differentiate to Thl cells by down-regulating transcription factor T-bet. Resveratrol also could inhibit Th2 cells differentiation from decreasing the expression of mRNA of GATA-3. And these two effects are all concentration related.

关 键 词：白藜芦醇 TH细胞 T-BET GATA-3 

分 类 号：R285.5[医药卫生—中药学]