Mutational analysis of the PTEN gene in soft tissue sarcomas  

抑癌基因PTEN突变在软组织肉瘤中的研究(英文)

作　　者：Liang Yin Yunzhao chen Wenting Li Benlin Hua Jinfang Jiang Hongan Li Feng Li 

机构地区：[1]Department of Pathology, The First Affiliated Hospital of Shihezi University School of Medicine, Shihezi 832002,China

出　　处：《The Chinese-German Journal of Clinical Oncology》2010年第7期422-425,共4页中德临床肿瘤学杂志（英文版）

基　　金：Supported by a grant from the National Natural Science Foundation of China (No. 30560169)

摘　　要：Objective： This study was to investigate whether PTEN mutations play a role in the carcinogenesis of soft tissue sarcomas （STS）. Methods： Polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to amplify 4 exons of PTEN and to analyze the conformation polymorphism, then DNA sequencing methods was used to detect point mutation of PTEN gene four exons of abnormal single strand conformation in soft tissue sarcomas. Results： Two of 86 cases showed 130th condon G→A missense mutation in the exon 8 of PTEN gene, and this mutation made Arg to change to Gin in PTEN protein structure 334th condon A→T missense mutation in the exon 8 of PTEN gene, and this mutation made Asn to change to Lys in PI-EN protein structure. Conclusion： These data indicated the existence of PTEN mutation in soft tissue sarcomas, but PTEN gene mutation rate is very low. PTEN mutation may prays an less important role in the development and malignant transformation of soft tissue sarcomas.

关 键 词：soft tissue sarcomas （STS） PTEN MUTATION 

分 类 号：Q253[生物学—细胞生物学] X591[环境科学与工程—环境工程]