霉酚酸对人髓样树突状细胞成熟、吞噬及刺激同种异体CD4＋T淋巴细胞增殖的作用  

Effect of mycophenolic acid on maturation, endocytotic capacity and allostimulatory properties of human myeloid dendritic cell

作　　者：侯静[1] 唐大年[2] 许媛[1] 韦军民[2] 贺修文[2] 李永国[3] 

机构地区：[1]首都医科大学附属北京同仁医院ICU,100730 [2]卫生部北京医院普外科 [3]中南大学湘雅二医院普外科

出　　处：《中华医学杂志》2010年第27期1910-1913,共4页National Medical Journal of China

摘　　要：目的 探讨霉酚酸（MPA）对人外周髓样树突状细胞（MDC）成熟、吞噬及刺激同种异体CD4＋T淋巴细胞增殖能力的影响.方法 获得健康志愿者外周血单个核细胞（n=15）,实验组加入MPA培养后,流式细胞仪分析MDC表面共刺激因子和黏附分子的表达水平;分离树突细胞抗原-1＋（BDCA-1＋）细胞,流式细胞仪测定BDCA-1＋细胞中FITC所标记的右旋糖酐的荧光值;通过混合淋巴细胞反应,流式细胞仪测定各组CD4＋T淋巴细胞在G0期的比例.结果 与对照组相比,MPA能明显降低实验组MDC表面CIMO、CD62L、HLA-DR、CD54、CD80、CD83和CD86的表达水平（t=-3.713,P=0.010）.MPA能明显增强实验组MDC的吞噬能力（t=10.171,P=0.000）.MPA几乎完全抑制实验组MDC诱导CD4＋T淋巴细胞分裂、增殖的能力.结论 MPA可增强人外周MDC的吞噬能力,抑制MDC的成熟和抑制MDC刺激同种异体CD4＋T淋巴细胞增殖的能力.Objective To study the effect of mycophenolic acid（MPA） on maturation, endocytotic capacity and allostimulatory properties of human mycloid dendritic cell （ MDC ）. Methods The peripheral blood mononuelear cells were isolated freshly from healthy volunteers （n = 15 ）. The study group was treated by MPA. Co-stimulatory and adhesion molecular on MDC were analyzed by flow eytometry. After isolation of blood dendritic cell antigen-1^＋ （ BDCA-1^＋ ） , the cellular uptake of FITC-dextran was measured by flow eytometry. After mixed lymphocyte reaction, the percentage of allogenie CD4^＋ T cell in Go phase was analyzed by flow eytometry. Results （ 1 ） Maturation ： Compared to control group, MPA down-regulated the level of CIM0, CD62L, HLA-DR, CD54, CD80, CD83 and CD86 on MDC significantly in study group （t = -3. 713 ,P 〉 0. 010）. （2） Endoeytotic capacity： MPA enchanced significantly the endocytotie capacity of MDC in study group （ t = 10. 171, P = 0. 000）. （ 3 ） Allostimulatory capacity： Compared to control group, MPA reduced the ability of MDC to stimulate markedly the T cell proliferation in study group. Conclusion MPA may enhance the phagocytic capacity of human peripheral MDC, inhibit the maturity of MDC and impair its allostimulatory capacity of CD4＋ T lymphocyte.

关 键 词：霉酚酸 次黄嘌呤类 T淋巴细胞 

分 类 号：R392[医药卫生—免疫学]