应变／应变率成像结合基质金属蛋白酶-9评价心力衰竭大鼠心肌收缩功能及心室重构  

Evaluation of the myocardial systolic function and ventricular remodeling of rats with experimental myocardial infarction by strain/strain rate imaging and MMP-9

作　　者：朱文晖[1] 段星星[1] 张梦玺[2] 唐水娟[1] 刘稳刚[1] 

机构地区：[1]中南大学湘雅三医院超声科,长沙410013 [2]中南大学湘雅三医院心血管内科,长沙410013

出　　处：《中华心血管病杂志》2010年第7期597-600,共4页Chinese Journal of Cardiology

基　　金：湖南省自然科学基金（09JJ6043）

摘　　要：目的 应用应变/应变率成像（S/SRI）及基质金属蛋白酶-9（MMP-9）蛋白表达评价心肌梗死后心力衰竭（心衰）大鼠心肌收缩功能及心室重构.方法 70只雄性SD大鼠随机分为4组：手术4周组及手术8周组,开胸结扎左冠状动脉前降支;假手术组,同样开胸,但不结扎;正常组作为对照.应用S/SRI定量评价心肌局部收缩功能,用Western blot法检测各组实验大鼠心肌MMP-9蛋白表达.结果 （1）除下壁中间段及基底段外,手术4周组各节段收缩期峰值应变、收缩期峰值应变率、收缩末期应变均明显下降（P〈0.05）,收缩后应变指数升高（P〈0.05）;手术8周组各指标值较手术4周组变化更明显（P〈0.05）.（2）手术4周组和手术8周组大鼠心肌MMP-9蛋白的表达明显增加（P〈0.05）,且手术8周组MMP-9蛋白的表达高于手术4周组（P〈0.05）.结论 S/SRI技术可定量检测心衰大鼠局部心肌收缩功能,在术后8周内大鼠心肌组织MMP-9蛋白的表达随心衰的进展而增高.Objective To evaluate the myocardial systolic function and ventricular remodeling in heart failure rat induced by myocardial infarction （MI） with S/SRI and MMP-9. Methods A total of 70 male SD rats were randomly assigned to 4 groups： 4 weeks and 8 weeks MI （ anterior descending branch of left coronary artery were ligated ） , sham operation （thoracotomy without ligation of coronary artery ） and nonoperated control group. The regional myocardial systolic function of rats was quantified with S/SRI. The myocardial MMP-9 expression was detected by Western blot Results In the 4 weeks MI group, all segment＇s Ssys, SRsys, the strain of end-systole were reduced while PSI was increased compared to sham and nomoperated group with the exception of the inferior wall. These changes were more significant in 8 weeks MI group compared to the 4 weeks MI group. In the 4 weeks MI group, the expression of MMP-9 was significantly upregulated than the sham operation group and this upregulation was more significant at 8 weeks post MI. Conclusions S/SRI can quantitative evaluate the regional systolic function of heart failure rat induced by myocardial infarction. Progressive upregulation of myocardial MMP-9 expression paralleled the deterioration of regional systolic function in this heart failure rat model.

关 键 词：心力衰竭 充血性 基质金属蛋白酶 超声心动描记术 多普勒 应变/应变率成像 

分 类 号：R541.6[医药卫生—心血管疾病]