东北地区J亚群禽白血病病毒的分子生物学特性分析  

Molecular Properties of Subgroup J Avian Leukosis Virus Isolated in Northeast China

作　　者：刘超男[1,2] 高玉龙[1] 景龙[2] 高宏雷[1] 祁小乐[1] 潘伟[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,哈尔滨150001 [2]东北农业大学,哈尔滨150030

出　　处：《畜牧兽医学报》2010年第7期915-921,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点实验室开放基金(SKLVBD200901);现代农业肉鸡产业技术体系建设(nycytx-42-G3-01)

摘　　要：为了解近两年在中国东北地区出现的J亚群禽白血病病毒(ALV-J)的分子生物学特性,作者从东北地区不同养禽场先后分离鉴定12株ALV-J,并对其env基因、3′UTR和3′LTR区序列进行测定和比较分析。结果显示,12株ALV-J分别位于env基因遗传进化的3个不同分支(group),其中group2和group3中毒株在env基因分别出现大片段氨基酸缺失和与其他亚群毒株重组现象;12株ALV-J在3′UTR区仍存在较大差异,具有完整E组件的ALV-J占主导地位;12株ALV-J在3′LTR区的变异主要集中于U3区,但是位于U3区的C/EBP、CArGbox、Y box、PRE box和TATA box等转录调控元件却高度保守。以上结果表明,目前中国ALV-J的基因变异仍主要集中在env基因和3′UTR区,且env基因已出现缺失或重组等新的变异趋势,并可能是ALV-J流行范围扩大、组织嗜性和致肿瘤作用明显改变的重要遗传基础。In order to analyze the variation status and molecular properties of subgroup J avian leukosis virus（ALV-J） in Northeast China,12 strains of ALV-J were isolated from commercial egg-type chickens suffering from hemangiomas and myelocytomas of the region.The complete env gene,3′untranslated region（3′UTR） and 3′ long terminal repeat（LTR） sequences were amplified,cloned and sequenced.Sequence analysis of the env gene,3′UTR and 3′LTR revealed several unique genetic characteristics of 12 recent isolates.Phylogenetic trees showed that the 12 isolates from different flocks fall into three separated groups.Among 12 isolates,the 3 isolates were classed into group2 showing 120 bp deletion in the env gene and 2 isolates were classed into group3 showing recombination between earlier ALV-J and endogenous virus sequence.In addition,the intact E element was found in majority of isolates and highly conserved;within the 3′LTR,the direct repeat region（R） and the 3′unique region 5（U5） were well conserved;albeit U3 region exhibited high mutations,the transcriptional regulatory elements including C/EBP,CArG box,Y box,PRE box and TATA box were highly conserved.These results suggested that the deletion and recombinant in envelope gene has became new variation tendency of ALV-J in China,and the variation of the envelope gene and 3′UTR were probably important in tissue tropism changes and induce tumors.

关 键 词：东北地区 J亚群禽白血病病毒 3′非编码区 转录调控元件 

分 类 号：S852.659.3[农业科学—基础兽医学]