蛋白酶激活受体-2基因mRNA在不同被毛颜色羊驼皮肤组织中的转录分析  

Expression Levels of PAR-2 mRNA in Skin of Alpacas with Different Coat Color

作　　者：姜俊兵[1] 于秀菊[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2010年第7期922-926,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30571070;30671512)

摘　　要：蛋白酶激活受体-2(Protease-activated receptor-2,PAR-2)是表达于角质化细胞的膜受体蛋白之一,经证实参与黑色素颗粒的胞间转运,本文通过分析PAR-2基因在不同毛色羊驼皮肤组织中的mRNA转录水平,以期从色素颗粒转运角度研究羊驼不同颜色被毛形成机制。以4头成年白色羊驼和4头棕色羊驼为研究对象,应用实时荧光定量PCR技术,对PAR-2在白色和棕色羊驼皮肤中的mRNA表达水平进行研究。结果显示,PAR-2基因mRNA在棕色羊驼皮肤组织中的相对基因表达量是白色羊驼的2.43倍。结果提示,PAR-2基因mRNA在白色和棕色羊驼皮肤组织中的表达差异表明:毛囊黑色素细胞与角质形成细胞间色素颗粒转运水平是羊驼不同被毛颜色形成的机制之一。Protease-activated receptor-2（PAR-2） is one of the receptor proteins on the membrane of keratinocyte which has been proved involving transportation of melanin granule among melanocyte and keratinocyte.To explore the mechanism of coat color formation of alpaca（Lama pacos）.The expression level of PAR-2 mRNA in skin of alpacas with different coat colors was analyzed.The expression levels of PAR-2 mRNA in skin of alpacas with different coat colors were examined by QRT-PCR.The expressive quantity of PAR-2 mRNA in the brown alpaca′s skin tissue was 2.43 times than ones of natural white alpaca.Based on the expression level analysis of PAR-2 mRNA,the result showed that the levels of melanin granules transportation could be one of the mechanisms of alpaca coat color formation.

关 键 词：蛋白酶激活受体-2 羊驼 皮肤组织 实时荧光定量PCR 

分 类 号：S852.161[农业科学—基础兽医学]