TRB3基因＋251A/G多态性与代谢综合征患者左房功能的相关性研究  

Association of the TRB3 + 251A /G polymorphism with left atrial dysfunction in patients with metabolic syndrome

作　　者：闫会敏[1] 杨静[2] 巩会平[1,3] 王志浩[1] 方宁宁[1] 王佳[1] 张薇[1] 张运[1] 钟明[1] 

机构地区：[1]山东大学齐鲁医院心内科,教育部和卫生部心血管重构和功能研究重点实验室,济南250012 [2]山东省威海市立医院保健科,山东威海264200 [3]青岛大学医学院附属医院特需保健科,山东青岛266003

出　　处：《山东大学学报（医学版）》2010年第7期69-72,76,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30871038、30971215);山东省自然科学基金资助项目(ZR2009CM025、Y2005C11);山东省科技攻关计划资助项目(2006GG2202020)

摘　　要：目的探讨TRB3基因+251A/G多态性与代谢综合征(MS)患者左房功能改变的相关性。方法选择MS患者177例,正常对照组156例。采用酚/氯仿法提取全血DNA,PCR-RFLP法检验受试者TRB3基因第二外显子+251A/G的多态性位点。应用应变率成像技术测量左房各壁的应变率。结果 +251A/G位点AA、AG+GG基因型和等位基因的频率分布在正常对照组和MS组,两组间差异有统计学意义(P均<0.05);与正常对照组相比,MS组左房平均收缩期应变率峰值(mean SSR)、平均舒张早期应变率峰值(mean ESR)显著降低(P均<0.001),提示MS患者左房储存器和管道功能显著减低,左房平均舒张晚期应变率峰值(mean ASR)差异无统计学意义(P>0.05),MS患者左房助力泵功能无显著变化;与AA基因型相比,AG+GG基因型者左房mean SSR、mean ESR和mean ASR均明显降低(P均<0.05);多元线性回归分析显示mean ESR、mean ASR与TRB3基因+251A/G多态性G等位基因之间存在数量依存关系(P<0.05)。结论 TRB3基因+251A/G多态性G等位基因与MS患者左房功能受损相关,在MS患者左房功能损害中可能起重要作用。Objective To investigate the association of the TRB3 gene ＋251A /G polymorphism with alterations of left atrial function in patietns with metabolic syndrome（MS）.Methods A total of 177 MS patients and 156 gender-and age-matched healthy subjects as controls were included.Genomic DNA was obtained from peripheral blood leukocytes using standard phenol-chloroform extraction procedures.The TRB3 gene ＋ 251A /G polymorphism was determined for all participants by the polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method.Strain rate imaging was preformed to assess left atrial function.Results The distributions of genotypic and allelic frequencies of the TRB3 gene ＋ 251A /G polymorphism showed significant differences between the MS group and controls（all P〈0.05）.Compared with controls,the decreases in mean SSR and ESR,reflecting impairment of the LA reservoir and conduit function in patients with MS,were significant（both P〈0.001）.However,mean ASR,an indicator of LA booster function,was similar in the two groups（P〈0.05）.This finding suggested there were no changes in the boost-er function of the left atrium.The carriers of the G allele（AG and GG genotypes） demonstrated significantly lower mean SSR,mean ESR and mean ASR of the left atrium than those in carriers of the AA genotype in the MS group（both P〈0.05）.Multiple linear regression analysis showed that the G allele of the TRB3 gene ＋ 251A/G polymor-phism significantly correlated with mean ESR and mean ASR of LA（both P〈0.05）.Conclusion The TBB3 gene ＋ 251A /G polymorphism has been associated with alterations of LA function.The G allele of the ＋ 251A /G polymor-phism could be an important risk factor for deteriorations of LA function in the patients with metabolic syndrome.

关 键 词：代谢综合征 TRB3基因多态性 左房功能 

分 类 号：R541.8[医药卫生—心血管疾病]