硅纳米颗粒的表面改性及生物应用  

Surface modification and biomedical application of silica nanoparticles

作　　者：赵颜忠[1,2] 余智萍[2] 朱晒红[2] 周建大[2] 黄艳艳[2] 王国慧[2] 黄东[2] 周科朝[1] 

机构地区：[1]中南大学粉末冶金国家重点实验室,长沙410083 [2]中南大学湘雅三医院,长沙410013

出　　处：《中国有色金属学报》2010年第7期1412-1417,共6页The Chinese Journal of Nonferrous Metals

基　　金：湖南省自然科学基金资助项目(06JJ50072);湖南省科学计划资助项目(05FJ3015)

摘　　要：以正硅酸乙酯为原料,通过添加氨基化试剂和钌吡啶配合物水溶液,采用油包水制备硅纳米颗粒、表面改性的硅纳米颗粒和荧光硅纳米颗粒。通过电镜检测到纳米颗粒的粒径约为40nm;在中性pH条件下,Zeta电位仪检测表面改性的硅纳米颗粒的净正电荷约为16mV;细胞内吞实验和体外毒性实验表明,荧光颗粒可被细胞吞噬,对细胞的生长无明显影响;与DNA的结合试验发现,氨基化硅纳米颗粒能与质粒DNA有效结合,复合后能有效地抵抗血清或DNaseI的降解;细胞转染实验表明,颗粒有效地将绿色荧光蛋白(GFP)基因转染到HT1080细胞和Hela细胞内,并高效表达。Silica nanoparticles, amino-terminated silica nanoparticles and fluorescent silica nanoparticles were prepared respectively via the formation of hydrolysis of tetraethyl orthosilicate （TEOS） with the synchronous modification of amino functional group in water-in-oil microemulsion. The diameter of the prepared nanoparticles was tested to be 40 nm through TEM analysis, and Zeta potential of the silica nanoparticles surface modified was tested to be 16 mV with zeta potentioneter under the condition of neutral pH value. Agarose gel electrophoresis photos indicate that the amino-terminated silica nanoparticles can bind effectively with DNA molecule, and moreover, nanoparticles-DNA complexes formed could resist digestion of DNase in the serum. The tests of cell by microscopy show that the most of nanoparticles can enter the cells when fluorescence nanoparticles are cultured with live HT1080 cells. Toxicity experiments in vitro indicate that there is no significant influence silica nanoparticles when they are cultured with normal cells. Finally, transfection tests of silica nanoparticles in the cultured cells reveal that plasmid DNA （pEGFP-N1_green fluorescence protein） can obtain expression with certain efficiency when the complexes formed with silica nanoparticles modified and DNA （pEGFP-N1） are cultured with Hela cells.

关 键 词：硅纳米颗粒 基因载体 细胞转染 基因治疗 表面改性 

分 类 号：TB39[一般工业技术—材料科学与工程]