人角膜基质成纤维细胞分泌作用与脂多糖的影响  

Effect of lipopolysaccharide on the secretion of human corneal stromal fibroblasts

作　　者：姜宏[1] 许海洋[2] 郝继龙[1] 

机构地区：[1]吉林大学中日联谊医院眼科,吉林省长春市130031 [2]吉林大学第一医院神经外科,吉林省长春市130011

出　　处：《中国组织工程研究与临床康复》2010年第28期5163-5166,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30772384);课题名称:Triptolide抑制角膜基质胶原降解及角膜溃疡的机制;吉林省科技发展计划资助项目(20090173;20090744);课题名称:TPCA-1对脂多糖诱导的角膜基质细胞分泌的抑制作用(20090173);细胞信号转导机制在角膜溃疡发生的作用(20090744)~~

摘　　要：背景:体外培养的角膜基质成纤维细胞,可以识别革兰阴性细菌细胞壁成分脂多糖,并释放多种细胞因子和趋化因子,从而促进角膜溃疡的形成。目的:观察细菌脂多糖对角膜基质成纤维细胞分泌作用的影响。方法:体外培养人角膜基质细胞,取第3～5代细胞鉴定后用于实验。采用ELISA法检测角膜基质细胞脂多糖作用前后培养上清中分泌白细胞介素1,肿瘤坏死因子α,白细胞介素6,白细胞介素8水平。结果与结论:脂多糖在一定质量浓度范围及作用时间内,对人角膜基质细胞增殖存活率没有影响。培养的人角膜基质成纤维细胞不表达白细胞介素1,肿瘤坏死因子α,未给予脂多糖的角膜基质细胞微量表达白细胞介素6,白细胞介素8,脂多糖(100μg/L)作用24h后,白细胞介素6,白细胞介素8水平明显增高。BACKGROUND： Corneal stromal fibroblasts cultured in vitro can recognize lipopolysaccharide, the main constituent of gram-negative bacterial cell wall, and release varied cytokines and chemotactic factors, which facilitate leukocytic infiltration and promote formation of corneal ulcer. OBJECTIVE： To observe the effect of bacterial lipopolysacchride on the secretion of human corneal stromal fibroblasts. METHODS： Human corneal stromal cells were cultured in vitro, and the 3-5 generations were selected for the experiment. The levels of interleukin 1, tumor necrosis factor α, interleukin 6 and interleukin 8 in supernatant of corneal stromal fibroblasts were determined by ELISA prior to and after adding lipopolysaccharide. RESULTS AND CONCLUSION： In a certain range of mass concentration and action times, lipopolysaccharide had no effect on survival rate of human corneal stromal fibroblasts. No interleukin 1 and tumor necrosis factor α were expressed in the cultured human corneal stromal fibroblasts. However, interleukin 6 and interleukin 8 were slightly expressed in human corneal stromal fibroblasts without lipopolysaccharide. At 24 hours after adding lipopolysaccharide （100 μg/L）, the levels of interleukin 6 and interleukin 8 were increased obviously.

关 键 词：角膜基质细胞 脂多糖 成纤维细胞 细胞培养 白细胞介素 

分 类 号：R318[医药卫生—生物医学工程]