兔不同程度椎体切除相邻椎间盘的组织学变化  

Histological changes in rabbit adjacent intervertebral disc following different degrees of vertebral body resection

作　　者：张亮[1] 靳安民[2] 高梁斌[1] 李健[1] 杨波[1] 张志[1] 王簕[1] 

机构地区：[1]广州医学院第三附属医院骨科,广东省广州市510150 [2]南方医科大学珠江医院骨科中心,广东省广州市510282

出　　处：《中国组织工程研究与临床康复》2010年第30期5631-5634,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广州市医药卫生科技项目(2009-YB-165)~~

摘　　要：背景:切除部分椎体是否可能会导致椎间盘营养通道的破坏,从而导致椎间盘的退行性变,目前很少有研究涉及。目的:评估兔椎体不同程度切除对相邻椎间盘退行性变的影响。方法:将10只新西兰兔分为2组,根据椎体不同程度切除植骨内固定,分为切除1/2椎体组和切除3/4椎体组。在造模后6个月处死动物,获取伤椎下位椎间盘及正常椎间盘组织(设为正常对照组);并进行苏木精-伊红染色,观察椎间盘组织学改变,检测椎体软骨终板血管芽数量;通过阿新蓝染色测量图像阳性吸光度以评价椎间盘髓核组织中蛋白多糖的变化。结果与结论:兔椎体切除小于1/2时,相邻椎间盘营养状况不会有明显改变;如果前路减压需切除椎体不超过1/2时应考虑单节段植骨融合双节段固定。椎体切除大于3/4时,相邻椎间盘营养状况会有明显改变,并导致椎间盘退行性变;如果前路减压需切除椎体超过3/4时应考虑切除相邻间盘行两节段植骨融合。BACKGROUND：It remains unclear whether partial vertebral body resection affects nutrition path of intervertebral disc,thereby leading to degeneration of intervertebral disc.OBJECTIVE：To investigate the impact of the degrees of vertebral body resection in rabbit on degeneration of adjacent intervertebral disc.METHODS：A total of 10 New Zealand rabbits were divided into two groups according to the degrees of resection of vertebral body（1/2 and 3/4）.At 6 months after operation,the intervertebral disc adjacent to the injured vertebrae and the normal intervertebral disc（normal control） were evaluated by means of hematoxylin-eosin staining to determine the number of vascular buds in Cartilage end-plate,and the changes of nucleus pulposus.The vertebral discs were also measured by alcian blue stained and calculated image optical density（IOD） to evaluate the changes of proteoglycan in nucleus pulposus.RESULTS AND CONCLUSION：When the removal of vertebral body was less than 1/2 in anterior decompression,the nutrition condition remained unchanged,so a single segment fusion with anterior bisegment fixation should be considered.If vertebral body resection was more than 3/4,the adjacent intervertebral disc exhibited significant changes in nutritional status and lead to intervertebral disc degeneration.Therefore,double segment fusion with anterior bisegment fixation should be considered.

关 键 词：椎间盘 椎体部分切除 退行性变 血管芽 组织学 

分 类 号：R318[医药卫生—生物医学工程]