重组人白细胞介素10抗家兔同种异体皮肤移植排斥模型的建立  

Construction of a recombinant human interleukin 10 against skin allograft rejection model

作　　者：冯继红[1] 孙万邦[1] 罗军敏[2] 刘凯山[1] 张磊[1] 温琼娜[1] 陈富超[2] 王胜香[1] 

机构地区：[1]遵义医学院珠海校区,广东省珠海市519041 [2]遵义医学院免疫学教研室,贵州省遵义市563003

出　　处：《中国组织工程研究与临床康复》2010年第31期5758-5762,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：贵州省教育厅重点培育项目(2008032)课题名称:重组人白细胞介素10生产技术及生物学功能研究~~

摘　　要：背景:研究表明,白细胞介素10在移植排斥反应的预防和治疗中具有一定的应用前景,有望成为一种新的临床用免疫抑制剂。动物移植模型是研究免疫抑制剂常用的实验平台,最常用的皮肤移植模型动物是小鼠,但小鼠属于小动物模型,移植技术要求较高。目的:建立家兔同种异体皮肤移植模型,观察重组人白细胞介素10抗家兔皮肤移植排斥反应的效果。方法:取家兔背部皮肤修剪成含真皮层的全厚皮片、中厚皮片和不含真皮层的薄层皮片进行家兔自体皮肤移植,随机分为固定组和非固定组,固定组用自制脖套对家兔头颈部活动进行一定限制;药物抗家兔同种异体移植排斥设重组人白细胞介素10组、环孢素A阳性对照组和生理盐水阴性对照组。采用混合淋巴细胞反应法观察家兔混合淋巴细胞反应增殖指数。在30d内连续观察移植皮肤出现排斥的时间和移植皮片平均存活时间。结果与结论:供、受体混合淋巴细胞培养呈增殖反应,表明家兔同种异体主要组织相容性复合体不同,相互皮肤移植可发生排斥反应。移植皮片厚度以含真皮的中厚皮片为最佳;自制脖套固定可有效限制家兔脖头颈部活动,有利于移植创面的保护。实验成功建立了重组人白细胞介素10抗家兔同种异体皮肤移植排斥模型,重组人白细胞介素10具有抗移植排斥的作用。BACKGROUND： Previous studies demonstrated that interleukin-10 （IL-10） has application prospect in preventing and treating allograft rejection, which may be served as a new immunosuppressant. Animal transplantation models provide platform for studying immunosuppressant. Mouse is widely used in establishing skin allograft model, which require highly transplantation technique. OBJECTIVE： To establish a model of skin allograft rejection in rabbits and to study the effects of recombinant human IL-10 （rhIL-10） against skin allograft rejection. METHODS： The skin flap from rabbit back was cut into three kinds of skin grafts： the full thickness flap with dermis, the middle thickness flap with dermis and the thin layer flap without dermis, which were transplanted into rabbits. The rabbits were randomly divided into the brake group and non-brake group, those in the former group was wore a self-made collar in order to restrict his head and neck. Models that drugs against rabbit allograft rejection were divided into three groups： rhIL-10 experimental group, CsA positive control group and negative control group. The mixed lymphocyte reactions were used to observe the proliferation index. The graft rejection time and the average survival time were continuously observed for 30 days. RESULTS AND CONCLUSION： The effects of the donors and recipients mixed on proliferation of T lymphocytes showed that allogeneic major histocompability complex were different in rabbits, and rejection may be occurred when skins were transplanted each other. The middle thickness skin graft was optimal. The self-made neck-collars could fix and protect the transplanted wounds and skin grafts. The rabbit model of rhIL-10 against skin allograft rejection was established successfully, and rIL-10 has anti-agraft rejection effect.

关 键 词：皮肤移植 人白细胞介素10 免疫抑制剂 固定 移植排斥 

分 类 号：R617[医药卫生—外科学]