小鼠胸腺形态结构的增龄性变化  

Age-related morphology and structure changes of mouse thymus

作　　者：刘仰斌[1] 李启华[1] 

机构地区：[1]赣南医学院,江西省赣州市341000

出　　处：《中国组织工程研究与临床康复》2010年第31期5805-5808,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:树突状细胞是体内最重要的抗原提呈细胞,在免疫调节中扮演着免疫应答和免疫耐受的双重角色,胸腺含有较多的树突状细胞。随着鼠龄增加,小鼠胸腺结构呈退化趋势,树突状细胞抗原提呈作用也在减弱。目的:观察不同年龄时期小鼠胸腺形态结构的变化。方法:分别观察出生后4周龄、20周龄及10月龄小鼠的胸腺结构;通过S100免疫组织化学标记,计算S100+胸腺树突状细胞在参照物(胸腺)中所占面积百分比;计算胸腺树突状细胞密度。结果与结论:随着年龄的增长,小鼠胸腺皮质变薄,皮髓质界限清楚。胸腺髓质由散在斑片状连成片。10月龄小鼠胸腺实质部分明显减少,而被充满脂肪细胞的间质所取代。20周龄小鼠的胸腺树突状细胞所占面积比和胸腺树突状细胞密度均比4周龄的增加,然而10月龄者则明显减少(P<0.01)。10月龄小鼠胸腺皮髓质交界处有较多S100+巨噬细胞。结果提示,随着鼠龄增加,小鼠胸腺结构呈退化趋势;老年鼠胸腺树突状细胞抗原提呈作用的减弱与胸腺树突状细胞数量的减少有密切关系。BACKGROUND： Dendritic cells are the most important antigen presenting cells in vivo and play a double role of immune response and immune tolerance. There are more dendritic cells in thymus. With the increasing age of mouse, structure of mouse thymus shows degradation tendency, and the role of antigen-presenting dendritic cells is also reduced. OBJECTIVE： To explore the morphologic change of different growth period mouse thymus. METHODS： The thymus structures of the 4-week-old, 20-week-old and 10-month-old mouse were observed; dendritic cells occupied area percentage in the thymus were observed and analyzed by S100 immunohistochemistry and the dendritic cells density was calculated. RESULTS AND CONCLUSION： Following age increasing, thymic cortex became thinning and the limit between cortex and medulla were more distinct. The glandular substance of 10-month-old mouse were obviously decreased and substituted with interstitial substance to be full of adipocytes. S100＋ dendritic cells area and dendritic cells density in 20-week-old mouse were significantly higher than in 4-week-old mouse, but were decreased obviously in 10-month-old mouse （P 0.01）. Simultaneously, there were more S100＋ macrophage on juncture between cortex and medulla in 10-month-old mouse thymus. Results demonstrated that, with age increasing, structure of mouse thymus trends to degradation; lower of antigen presenting effect of dendritic cells in gerontism mouse were closely related to reduction of quantity of dendritic cells.

关 键 词：胸腺树突状细胞 小鼠 胸腺 增龄 形态学 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]