苯肼致小鼠溶血性贫血模型的建立  

Establishment of an Acute Hemolytic Anemia Mouse Model Induced by Phenylhydrazine and its Potential Applications in Regenerative Medicine

作　　者：王若永[1] 习佳飞[1] 何丽娟[1] 谢小燕[1] 杨超[1] 陈琳[1] 李艳华[1] 裴雪涛[1] 

机构地区：[1]军事医学科学院野战输血研究所干细胞与再生医学研究室,北京100850

出　　处：《生物技术通讯》2010年第4期544-546,560,共4页Letters in Biotechnology

基　　金：国家高技术研究发展计划重大项目(2006AA02A107);北京市科委科技计划研发攻关类重大项目(D07050701350702)

摘　　要：目的:应用苯肼致小鼠发生溶血性贫血,建立急性溶血性贫血模型,筛选苯肼致小鼠贫血最佳浓度和红细胞移植的最佳时机。方法:30只C57BL/6小鼠随机分为6组,经腹腔注射不同浓度的苯肼溶液,于注射前和注射后第1、3、5、7、9天采集小鼠外周血进行检测,记录相关指标的变化,比较各组之间的差异,筛选出最佳溶血效果的给药浓度和红细胞移植治疗介入的时机。结果:注射苯肼溶液可使C57BL/6小鼠短期内产生明显的急性溶血性贫血症状,皮肤黏膜颜色苍白;外周血红细胞数量、血红蛋白含量、红细胞压积降低;随着苯肼溶液浓度的增加,小鼠体重显著减轻,存活率下降。结果表明,苯肼注射小鼠致贫血的最佳作用浓度为1.2mg/10g体重,小鼠贫血状态可维持7d。结论:建立了小鼠溶血性贫血模型,此模型可应用于红细胞输注效果的评价。Objective： To establish a model of anemia in mice induced by phenylhydrazine. Methods： Thirty C57BL / 6 mice were randomly separated into 6 groups, each group was injected with different dose of phenylhydrazine. Blood samples were harvested from tail vein at day 1, 3, 5, 7, 9 post injections and tested for red blood cell（RBC）, hematoglobin（HGB）, hematocrit（Hct）. Results： The anemia syndromes in group 120 and 160 mg / kg body weight（bw） were more serious than those in group 40 and 80 mg / kg bw. The RBC, HGB and Hct in C57BL / 6 were soon decreased after the injection of phenylhydrazine. Anemia could sustain about 6 days. Conclusion： The mouse model of acute hemolytic anemia by phenylhydrazine had been successfully established. The best effect was observed in group 120 mg / kg bw.

关 键 词：急性溶血性贫血 苯肼 动物模型 红细胞 

分 类 号：R556.6[医药卫生—血液循环系统疾病]