锌缺乏导致小鼠睾丸游离锌离子和附睾精子数量减少  

Zinc deficiency induced the reduction of zinc content in mouse testis and spermatozoa in mouse epididymis

作　　者：王玥[1,2] 武剑华[3] 王旭[1,2] 张浩[4] 池志宏[1] 王占友[1] 

机构地区：[1]中国医科大学基础医学院细胞工程研究室,辽宁沈阳110001 [2]辽宁中医药大学组织胚胎学教研室,辽宁沈阳110001 [3]中国医科大学 [4]沈阳医学院奉天医院第二神经外科,辽宁沈阳110024

出　　处：《解剖科学进展》2010年第4期347-349,共3页Progress of Anatomical Sciences

基　　金：辽宁省教育厅2009年高等学校科研项目计划(NO2009A749);辽宁省教育厅高等学校科研项目计划(No20060948)

摘　　要：目的研究锌缺乏对小鼠睾丸游离锌离子和附睾精子数量的影响。方法锌缺乏饲养小鼠5周后,应用ZnSe金属自显影技术对小鼠睾丸和附睾进行染色,观察睾丸和附睾锌离子的分布,同时计数附睾精子数量,并与对照组小鼠的精子数量做对比。结果缺锌喂养后的小鼠睾丸游离锌离子明显减少,并且睾丸生精小管管腔狭小,生殖细胞层厚度增加,此外,附睾精子数量也明显低于对照组。结论睾丸和附睾内游离锌离子减少是锌缺乏小鼠精子数量下降的原因,本结果有助于改善男性生殖健康的状况。Objective The aim of this research is to study the effect of zinc deficiency on the zinc ions in testis and the quantity of sperm in epididymis. Method The mice were given zinc deficiency food for 5 weeks, the distribution of zinc ions in normal and zinc deficiency mouse testis and epididymis, and the quantity of sperm in epididymis were detected by autometallography. Results Compared with the control group, zinc ions in number were decreased significantly in zinc deficiency mouse testis with narrow lumens of seminiferous tubule and thick generative cell layer. The number of sperm in epididymis was decreased in zinc deficiency mouse than in control. Conclusion The reduction of Zinc ions in the zinc deficiency mouse testis and epididymis causes the sperm degression, which is helpful for the improvement of the germinal condition.

关 键 词：锌离子 睾丸 附睾 小鼠 

分 类 号：R697.22[医药卫生—泌尿科学]