高眼压对青光眼视网膜功能的影响  

Effect of ocular hypertension on the function of retina of glaucoma

作　　者：郭学谦[1] 田蓓[2] 孙世杰[1] 谢楠[3] 刘志成[1] 

机构地区：[1]首都医科大学生物医学工程学院,北京100069 [2]首都医科大学附属北京同仁医院,北京100041 [3]国家知识产权局专利审查协作中心,北京100083

出　　处：《医用生物力学》2010年第3期195-199,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目(30600150;10802053;30670514);北京市教育委员会科技发展计划项目(KZ200710011010;KM200910025007);北京市属市管高等学校人才强教计划资助项目

摘　　要：目的研究青光眼持续高眼压对大鼠动物模型视网膜功能的影响。方法制备青光眼高眼压大鼠动物模型并定时监测眼压;于术前和术后3w、6w进行闪光视网膜电图测量和形态学观察。结果动物模型术后1d眼压升高到最大值(3.07依1.10)kPa,术后1w至6w各时段眼压值分别为:(2.84依0.90)kPa,(2.86依0.54)kPa,(2.76依0.52)kPa,(2.70依0.51)kPa,(2.39依0.27)kPa,(2.09依0.39)kPa,均明显高于对侧眼眼压;造模3w、6w时的视网膜电图a波、b波及振荡电位波的幅值等时域参数均显著降低,且随高眼压的持续呈现进行性下降;常规HE染色,没有发现视网膜有明显的组织学和形态学改变。结论慢性高眼压在造成视网膜组织学和形态学发生改变之前视网膜的光学功能已经受损,且损伤随高眼压的持续呈现进行性趋势。Objective To explore the effect of elevated intraocular pressure（IOP） on retina＇s function of rat.Method Chronic glaucoma rat models were built and IOP were measured persistently.Flash electroretinogram（F-ERG） and histological status were measured before and after surgery.Results The maximal IOP in rats without anesthesia is（3.07±1.10） kPa in 1 day after surgery.From 1 w to 6 w after surgery,the model-eye-IOPs are（2.84±0.90） kPa,（2.86±0.54） kPa,（2.76±0.52） kPa,（2.70±0.51） kPa,（2.39±0.27） kPa and（2.09±0.39） kPa,respectively,which are significant higher than that of the fellow eye.A series of main parameters including the amplitudes of a-wave,b-wave and oscillatory potentials（OPs） all dramatically decreased in model eyes at 6 weeks and 3 weeks postoperatively.No obvious changes were observed by routine HE staining among the eyes before and after surgery.Conclusions The chronic high intraocular pressure could induce the retinal dysfunction before any morphological change occurred,and what＇s more,the damage showed itself a progressive one.

关 键 词：眼压 青光眼 闪光视网膜电图 振幅 组织学 形态学 

分 类 号：R318[医药卫生—生物医学工程]