不同免疫途径对Sj童虫细胞型免疫原诱生的保护性效果及抗体应答差异  

Comparison of the immune protective effect and antibody response of primary cells from schistosomulum by two different immunization routes in mice

作　　者：刘碧源[1,2] 曾庆仁[1] 余平[1] 李艳琴[1] 魏琦[1] 杨胜辉[1] 刘彦[1,2] 张顺科[1] 蔡力汀[1] 

机构地区：[1]中南大学湘雅医学院免疫学系及细胞与分子生物学实验中心,长沙410013 [2]南华大学公共卫生学院及医学院,衡阳421001

出　　处：《中国人兽共患病学报》2010年第7期615-617,623,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(30570952)资助

摘　　要：目的比较研究小鼠经不同途径接种日本血吸虫(Schistosoma japonicum,Sj)童虫原代细胞(primary juvenile worm cells,pJCs)后所诱导的免疫保护效果,寻找其合适的免疫途径。方法将pJCs经皮下或静脉注射途径免疫昆明小鼠,间隔2w共免疫3次,末次免疫后第4w,与对照组同时经腹部皮肤感染尾蚴30±2尾/鼠,比较三组小鼠的肝脏虫卵数及虫卵肉芽肿的大小、成虫数、虫体大小。同时在第2、3次免疫前及攻击感染前1d,小鼠尾静脉采血,用ELISA法检测抗pJCs-IgG水平。结果 pJCs免疫小鼠后,与PBS对照组比,静脉免疫组的减虫率为48.53%、肝卵减少率为54.54%、虫体平均重量减少率为29.62%(P<0.01)、虫卵肉芽肿面积减少率为36.8%,而皮下免疫组分别为41.28%、43.19%、23.08%、31.2%。IgG抗体水平也是静脉注射组高(PBS组,P<0.01;皮下注射组,P<0.05)。结论日本血吸虫童虫细胞免疫原通过皮下和静脉注射均可诱导小鼠产生对日本血吸虫的免疫保护力,其中静脉注射诱导的免疫保护效应最强,提示通过静脉注射日本血吸虫童虫细胞是可行有效的免疫途径。In order to observe the influence of immunization route on the immune protective effects induced by primary juvenile worm cells（pJCs）,Kunming mice were inoculated intravenously or subcutaneously with pJCs from 12 day-old Schistosoma japonicum respectively.All mice were immunized three times at 2 weeks interval and were challenged each with 30±2 cercariae by abdominal skin penetration 4 weeks after the last booster.Six weeks of post-infection,mice were sacrificed by aether inhalation,and the numbers of worms and hepatic eggs were counted.Specific anti-pJCs levels in the mice sera collected one day before the second,third immunization and challenge infection were tested with ELISA.Mice immunized with pJCs given i.v.or s.c.exhibited high level of specific antibody and protection effects,but given i.v.was significantly higher than given s.c.in anti-pJCs level（P〈0.05）and showed significant reduction in worm burden（48.53%）,liver eggs per gram（LEPG）load（54.54%）,worm average weight（29.62%）and egg granuloma size（36.8%）.The juvenile worm cells of S.japonicum could induce partial protective immunity against S.japonicum in mice and the protective immunity by i.v.was better than that by s.c..These results indicated intravenous route was a feasible and efficacious route for juvenile worm cell immunization.

关 键 词：日本血吸虫 童虫原代细胞 免疫应答 免疫保护 

分 类 号：R383.2[医药卫生—医学寄生虫学]