成年期甲减对大鼠额叶SNAP-25蛋白表达的影响  

Effect of hypothyroidism on the SNAP-25 expression in the prefrontal lobe of adult rats

作　　者：杨会云[1] 王茜[1] 孙春萍 桂丽[3] 朱德发[1] 

机构地区：[1]安徽医科大学第一附属医院老年内分泌科,合肥230022 [2]安徽医科大学第三附属医院内分泌科,合肥230061 [3]安徽医科大学综合实验室,合肥230032

出　　处：《安徽医科大学学报》2010年第4期471-474,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30772301);安徽省自然科学基金(编号:070413084)

摘　　要：目的研究成年期甲状腺功能减退症(甲减)大鼠额叶内突触相关蛋白SNAP-25的表达,探讨甲减脑损伤的分子机制。方法用丙基硫氧嘧啶(PTU)建立成年期大鼠甲减模型,采用放射免疫法测定甲减组和正常对照组大鼠的血清甲状腺素水平,免疫组化方法分析两组大鼠额叶内SNAP-25蛋白的表达情况。结果甲减组大鼠血清T3、T4水平显著低于正常对照组(P<0.01、P<0.05);额叶Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ层SNAP-25蛋白的表达水平显著高于正常对照组(P<0.05、P<0.01)。结论成年期甲减大鼠额叶SNAP-25蛋白表达增多。Objective To observe the expression of synaptosome-associated protein of 25 ku （SNAP-25） in prefrontal lobe of adult-onset hypothyroidism rats and to explore the molecular mechanism of brain.Methods All 30 adult male Sprague-Dawley rats were separated into 2 groups randomly：Hypothyroidism group（n=15） and control normal group（n=15）.The hypothyroidism models were replicated with 6-n-propyl-2-thiouracil（PTU）.The levels of serum T3,T4 were assayed by radioimmunoassay method,and the level of SNAP-25 in prefrontal lobe was analyzed by immunohistochemistry.Results In the hypothyroidism group,the levels of serum T3 and T4 were significantly lower than those in the normal control group（P〈0.05）;and the levels of SNAP-25 protein in theⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ layers of prefrontal lobe were significantly higher than the normal control group（P〈0.05）.Conclusion The expression of SNAP-25 in the prefrontal lobe of adult-onset hypothyroidism can be increased.

关 键 词：甲状腺功能减退症 额叶 免疫组织化学 突触 锥体细胞 

分 类 号：R581.2[医药卫生—内分泌] R322.81[医药卫生—内科学]