重稀碱金属Cs^+跨人红细胞膜行为的研究(英文)  

The Uptake and Membrane Transport of Cesium in Human Erythrocytes

作　　者：卢靖[1] 黄剑锋[1] 

机构地区：[1]陕西科技大学教育部轻化工助剂化学与技术重点实验室,西安710021

出　　处：《无机化学学报》2010年第8期1349-1354,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.50772063);陕西科技大学研究生创新基金资助项目

摘　　要：采用原子吸收光谱法检测体外人红细胞摄取Cs+的含量,系统讨论了胞外Cs+浓度,温育时间、温育温度、介质pH值对人红细胞摄取Cs+过程的影响。选用不同离子通道或离子载体的特异性抑制剂进一步探讨Cs+的跨膜途径和机理。结果显示,各实验参数对人红细胞摄取Cs+均有一定的促进作用。Cs+主要借助Na+/K+-泵的主动运输方式跨膜;少量的Cs+能"漏入"细胞,微量的Cs+可以模拟Na+/Li+-反向协同运输的方式跨膜;在允许HCO3-存在的pH环境下,少量Cs+以Cl-/CsCO3-交换的形式通过膜上带3蛋白进入人红细胞;Ca2+通道对Cs+没有通透作用。The uptake of Cs^＋ by human erythrocytes in vitro was investigated via atomic absorption spectrometry. The results indicate that the metal ions concentration,incubation temperature,incubation hours and media pH all have a positive effect on this cellular uptake process. The mechanism of Cs^＋ transport into human erythrocytes was briefly discussed. There are mainly four pathways involve in the Cs^＋ transmembrane activity as far as we concerned：（1） an active transport system mediated by the Na^＋/K^＋-ATPase; （2） ＂leak＂ process through pores in the membrane proteins; （3）Na ＋-Cs ＋ countertransport systems; （4）Cl^-/CsCO3^-exchanger pathway stimulated by bicarbonate through anion channel （band 3 protein）. No inhibition but a little stimulation by Nifedpine in the Cs^＋ uptake demonstrates that the transport of Cs^＋ through Ca^2＋ channel is unavailable.

关 键 词：铯 人红细胞 摄取 膜运输 

分 类 号：O614.115[理学—无机化学] Q241[理学—化学]